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Title:SINGLE-SHOT SUB-MICROSECOND SPECTROSCOPY OF THE BACTERIORHODOPSIN PHOTOCYCLE WITH QUANTUM CASCADE LASER FREQUENCY COMBS
Author(s):Mangold, Markus
Contributor(s):Faist, Jerome; Allmendinger, Pitt; Hugi, Andreas; Klocke, Jessica; Kottke, Tilman
Subject(s):Mini-symposium: Frequency-Comb Spectroscopy
Abstract:Time-resolved vibrational spectroscopy is an important tool for understanding biological processes and chemical reaction pathways [1]. Today, all available methods to our knowledge require many repetitions of an experiment to acquire a microsecond time-resolved mid-infrared spectrum. We present the IRspectrometer, a quantum cascade laser dual frequency comb spectrometer [2-3]. It allows for parallel acquisition of hundreds of mid-infrared wavelengths with microsecond time resolution. The formation of the light-activated L, M and N-states in bacteriorhodopsin – which only have $\mu$s to ms lifetimes – has been recorded by analyzing the infrared response of bacteriorhodopsin to 10 ns visible light pulses with sub-microsecond time-resolution. The different wavelengths were all measured in parallel thanks to the dual-comb approach. The spectra as well as the kinetics show good agreement with those from step-scan FT-IR measurements. As a benchmark, the spectral signature of several intermediate states of the bacteriorhodopsin photocycle has been recorded in a single shot measurement. This approach greatly reduces the complexity of time-resolved spectroscopy measurements in the mid-infrared which currently require many repetitions. References [1] Ritter, E. et al. “Time-Resolved Infrared Spectroscopic Techniques as Applied to Channelrhodopsin.” Front. Mol. Biosci. 2:38. [2] Hugi, A. et al. “Mid-Infrared Frequency Comb Based on a Quantum Cascade Laser.” Nature 492: 229–33 [3] Villares, G. et al. “Dual-comb spectroscopy based on quantum-cascade-laser frequency combs.” Nat. Commun. 5:5192
Issue Date:06/19/18
Publisher:International Symposium on Molecular Spectroscopy
Citation Info:APS
Genre:Conference Paper / Presentation
Type:Text
Language:English
URI:http://hdl.handle.net/2142/100785
DOI:10.15278/isms.2018.TG03
Other Identifier(s):TG03
Date Available in IDEALS:2018-08-17
2018-12-12


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