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Title:Kinetics and mechanisms of bacteriophage PR772 inactivation by monochloramine
Author(s):Cong, Wen
Advisor(s):Mariñas, Benito J.
Department / Program:Civil & Environmental Eng
Discipline:Environ Engr in Civil Engr
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):bacteriophage PR772
drinking water
Abstract:With billions of people living with unsafe drinking water sources, ensuring safe drinking water remains a challenging task for environmental engineers and researchers. Human Adenovirus (HAdV) is a pathogen listed in the Contaminant Candidate List 4 of the United States Environmental Protection Agency. HAdV is highly resistant to monochloramine, a common drinking water disinfectant used in the United States and other countries around the world. PR772 is believed to relate evolutionarily to HAdV, which makes PR772 a potential surrogate virus for HAdV. Previous research characterized the monochloramine inactivation kinetics of both viruses, and found that the inactivation kinetics at high pH and high temperature was characterized by a phase of slower rate followed by faster rate, which was different from the more common fast-slow or tailing kinetics observed for other pH-temperature combinations, for both viruses as well as for other microorganisms. This thesis further characterized the kinetics of PR772 inactivation by monochloramine at high pH and high temperature, and proposed a two-reaction model to describe the data. Molecular analyses at various stages of the replication cycle were used to determine which viral replication step was hindered by monochloramine disinfection at pH 8.0-30.0℃ (baseline condition) and pH 10.5-30.0℃ (high pH and high temperature condition). The results showed that early mRNA synthesis, DNA production and late mRNA synthesis were all inhibited. Different from previous research on lower temperature and pH, partial genome damage and loss in viral attachment were observed. No difference between the two pH levels investigated was observed.
Issue Date:2018-04-10
Rights Information:Copyright 2018 Wen Cong
Date Available in IDEALS:2018-09-04
Date Deposited:2018-05

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