Files in this item

FilesDescriptionFormat

application/pdf

application/pdfGRANT-THESIS-2018.pdf (925kB)
(no description provided)PDF

video/avi

video/aviFrozen Differen ... Electrical Stimulation.avi (2MB)
(no description provided)AVI video

video/avi

video/aviFrozen Undiffer ... Electrical Stimulation.avi (2MB)
(no description provided)AVI video

video/avi

video/aviFrozen Undifferentiated Spontaneous.avi (2MB)
(no description provided)AVI video

video/avi

video/aviUnfrozen 1 Hz Electrical Stimulation.avi (2MB)
(no description provided)AVI video

Description

Title:Cryopreservation of tissue engineered skeletal muscle
Author(s):Grant, Lauren
Advisor(s):Bashir, Rashid
Department / Program:Bioengineering
Discipline:Bioengineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:M.S.
Genre:Thesis
Subject(s):biobot
bioactuator
skeletal muscle
tissue engineering
cryopreservation
Abstract:Tissue engineered skeletal muscle plays an important role not only in the field of regenerative medicine, but also in emerging areas such as soft robotics, organ-on-a-chip models of disease, and drug testing. However, further expansion of the applications of tissue engineered skeletal muscle models will require a suitable method for their long-term storage and shipment. Cryopreservation has long been the standard for long-term cell storage, but when it comes to the freezing of 3D tissues, many complications arise due to heat and mass transfer limitations. Here, we use a tissue engineered skeletal muscle bioactuator as a model to characterize the effects of freezing on skeletal muscle viability, gene expression, myotube structure, and force generation. We optimize the freezing medium composition and compare the effects of freezing on both undifferentiated and differentiated engineered skeletal muscle tissue constructs. We report an optimized protocol of freezing skeletal muscle constructs while undifferentiated, which not only maintains cell viability, but leads to a 3-fold increase in force production as compared to unfrozen muscle. The reported timeline for skeletal muscle tissue fabrication, freezing, and revival not only promotes a more streamlined fabrication process, but will further enable collaborative research efforts through the shipment of pre-formed skeletal muscle constructs.
Issue Date:2018-07-02
Type:Thesis
URI:http://hdl.handle.net/2142/101509
Rights Information:Copyright 2018 Lauren Grant
Date Available in IDEALS:2018-09-27
Date Deposited:2018-08


This item appears in the following Collection(s)

Item Statistics