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Title:Protein interactions in vitro and in the cell
Author(s):Guin, Drishti
Director of Research:Gruebele, Martin
Doctoral Committee Chair(s):Gruebele, Martin
Doctoral Committee Member(s):Leckband, Deborah; Freeman, Brian; Shukla, Diwakar
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Protein binding, in cell, in vitro, dodine, denaturant, protein interactions
Abstract:The cell is a complex crowded environment containing up to 300 mg/mL bio-macromolecules. In this environment proteins are far from isolated systems and interact with many other biological molecules they encounter. In recent years, better instrumentation and greater computing power have enabled imaging of elusive biomolecule dynamics in cells, driving many advances in understanding the chemical organization of biological systems. Interactions in the cell affect both biomolecular stability and function. The same protein or nucleic acid can behave differently depending on time in the cell cycle, location in a specific compartment, or stresses acting on the cell. My thesis focuses on these interactions both in vitro and in the cell and the differences between a model in vitro environment and the cell. The broader goal of my research is to emphasize the importance of the cellular environment in designing future biological studies.
Issue Date:2019-08-23
Type:Text
URI:http://hdl.handle.net/2142/106142
Rights Information:Copyright 2019 Drishti Guin
Date Available in IDEALS:2020-03-02
Date Deposited:2019-12


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