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Title:Holographic fast gradient light interference microscopy (HF-GLIM)
Author(s):Sakakura, Masayoshi
Contributor(s):Popescu, Gabriel
Subject(s):Holography
Quantitative phase imaging
Sagnac interferometer
Gradient light interference microscopy
Differential interference contrast microscopy
Abstract:Quantitative phase imaging has been an emerging technique in the field of biomedical imaging. As one type, gradient light interference microscopy (GLIM) is used to retrieve a quantitative gradient mapping of the phase delay that is produced by a biological sample. The method involves collecting four phase-shifted frames for a single field of view, which ultimately combines arithmetically into phase gradient information. The phase-shifting is done by a space light modulator (SLM) which intakes an appropriate voltage that converts into a corresponding phase retardation in the image field. Despite GLIM’s ability to generate a quantitative phase gradient of a sample, such module depends heavily on the operational speed of the SLM. The holographic fast gradient light interference microscopy (HF-GLIM) aims to retrieve a field of view with only a single image from the conventional differential interference contrast microscope (DIC), and is sent to a tilted Sagnac interferometer to create a holographic output at the camera. Through digital processing of the Hologram using Fourier filtering, the phase gradient that is given by the sample can be retrieved only through a single recording instance from the HF-GLIM. The publication aims to derive the functionality of HF-GLIM utilizing a mathematical derivation, geometrical analysis, and the simulation results.
Issue Date:2020-05
Genre:Other
Type:Text
Language:English
URI:http://hdl.handle.net/2142/107235
Date Available in IDEALS:2020-06-11


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