Files in this item

FilesDescriptionFormat

application/pdf

application/pdfFARMER-THESIS-2020.pdf (2MB)Restricted Access
(no description provided)PDF

Description

Title:High-throughput screening for ADP-ribosyl hydrolase 3 inhibitors
Author(s):Farmer, Rachel Elizabeth
Advisor(s):Hergenrother, Paul J.
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:M.S.
Genre:Thesis
Subject(s):ARH3
PAR
Screen
Abstract:Poly(ADP-ribose) (PAR) signaling is an important component of DNA damage repair, DNA replication, and initiation of parthanatos, a method of controlled cell death. While PAR has been well studied for decades, much is still unknown about PAR’s initiation of parthanatos and the importance of mono(ADP-ribose) (MAR) in cellular regulation. The lack of knowledge in this field can be attributed in part to the lack of a cell-permeable tool compound to study the activity of ARH3, a key degrader of PAR and MAR. Toward filling this need, a high-throughput screen was conducted using a fluorogenic substrate to discover an inhibitor of ARH3. A total of 496 primary hits have been identified and will be further studied to validate their ability to inhibit ARH3.
Issue Date:2020-12-08
Type:Thesis
URI:http://hdl.handle.net/2142/109637
Rights Information:Copyright 2020 Rachel Farmer
Date Available in IDEALS:2021-03-05
Date Deposited:2020-12


This item appears in the following Collection(s)

Item Statistics