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Title:Cellular thermometry considerations for probing biochemical pathways
Author(s):Rajagopal, Manjunath C., and Sinha, Sanjiv
Subject(s):intracellular thermometry
design of experiments
thermal conductivity
heat diffusion
Abstract:Temperature is a fundamental thermodynamic property that can serve as a probe of biochemical reactions. Extracellular thermometry has previously been used to probe cancer metabolism and thermoregulation, with measured temperature changes of ~1-2 K in tissues, consistent with theoretical predictions. In contrast, previous intracellular thermometry studies remain disputed due to reports of >1 K intracellular temperature rises over 5 min or more that are inconsistent with theory. Thus, the origins of such anomalous temperature rises remain unclear. An improved quantitative understanding of intracellular thermometry is necessary to provide a clearer perspective for future measurements. Here, we develop a generalizable framework for modeling cellular heat diffusion over a range of subcellular-to-tissue length scales. Our model shows that local intracellular temperature changes reach measurable limits (> 0.1 K) only when exogenously stimulated. On the other hand, extracellular temperatures can be measurable (> 0.1 K) in tissues even from endogenous biochemical pathways. Using these insights, we provide a comprehensive approach to choosing an appropriate cellular thermometry technique by analyzing thermogenic reactions of different heat rates and time constants across length scales ranging from sub-cellular to tissues. Our work provides clarity on cellular heat diffusion modeling and on the required thermometry approach for probing thermogenic biochemical pathways.
Issue Date:2021-04-02
Publisher:Springer Nature
Citation Info:Rajagopal, M.C., Sinha, S. Cellular Thermometry Considerations for Probing Biochemical Pathways. Cell Biochem Biophys (2021).
Rights Information:NSF-CBET-17-06854
Date Available in IDEALS:2021-04-05

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