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Title: | Cellular thermometry considerations for probing biochemical pathways |
Author(s): | Rajagopal, Manjunath C., and Sinha, Sanjiv |
Subject(s): | intracellular thermometry
design of experiments metabolism thermal conductivity heat diffusion |
Abstract: | Temperature is a fundamental thermodynamic property that can serve as a probe of biochemical reactions. Extracellular thermometry has previously been used to probe cancer metabolism and thermoregulation, with measured temperature changes of ~1-2 K in tissues, consistent with theoretical predictions. In contrast, previous intracellular thermometry studies remain disputed due to reports of >1 K intracellular temperature rises over 5 min or more that are inconsistent with theory. Thus, the origins of such anomalous temperature rises remain unclear. An improved quantitative understanding of intracellular thermometry is necessary to provide a clearer perspective for future measurements. Here, we develop a generalizable framework for modeling cellular heat diffusion over a range of subcellular-to-tissue length scales. Our model shows that local intracellular temperature changes reach measurable limits (> 0.1 K) only when exogenously stimulated. On the other hand, extracellular temperatures can be measurable (> 0.1 K) in tissues even from endogenous biochemical pathways. Using these insights, we provide a comprehensive approach to choosing an appropriate cellular thermometry technique by analyzing thermogenic reactions of different heat rates and time constants across length scales ranging from sub-cellular to tissues. Our work provides clarity on cellular heat diffusion modeling and on the required thermometry approach for probing thermogenic biochemical pathways. |
Issue Date: | 2021-04-02 |
Publisher: | Springer Nature |
Citation Info: | Rajagopal, M.C., Sinha, S. Cellular Thermometry Considerations for Probing Biochemical Pathways. Cell Biochem Biophys (2021). https://doi.org/10.1007/s12013-021-00979-w |
Genre: | Article |
Type: | Text |
Language: | English |
URI: | http://hdl.handle.net/2142/109735 |
DOI: | 10.1007/s12013-021-00979-w |
Rights Information: | NSF-CBET-17-06854 |
Date Available in IDEALS: | 2021-04-05 |
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Illinois Research and Scholarship (Open Community)
This is the default collection for all research and scholarship developed by faculty, staff, or students at the University of Illinois at Urbana-Champaign