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Title:Defluorination of PFOA and PFOS alters their toxicity in a hepatocyte and hepatic stellate cell co-culture system
Author(s):Wen, Yi
Subject(s):per- and polyfluoroalkyl substances
Abstract:Presented by: Yi Wen – Graduate Student at University of Illinois at Urbana-Champaign, Co-authors: Saeed Ahmad, Hyeon Ryoo, Zekun Liu, Jinyong Liu, Gregory H. Underhill, Joseph Irudayaraj Abstract: Perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) are two of the most widely used fluorosurfactants in the global industry. They are extremely persistent in the environment and have long half-life in humans. Recent efforts have been made to degrade or defluorinate long-chain perfluoroalkyl substances (PFAS) to reduce their toxicity. But little biological validation has been performed to demonstrate the reduction in toxicity of these defluorinated chemicals. A co-culture system of human hepatic stellate cells and spheroids formed of hepatocellular carcinoma cell HepG2 was designed to mimic human liver in vitro. This system was used as a model to study the toxicity of defluorinated PFOA and PFOS versus their intact forms. Both types of cells are evaluated on the different aspects of the PFAS toxicity, including cytotoxicity, metabolic toxicity, and epigenetic toxicity. Biography: Yi is a Ph.D. candidate in Dr. Joseph Irudayaraj Lab. He holds a M.S. in Bioengineering from University of Illinois as well as a B.S. in Biological Engineering and a B.S. in Biochemistry from Purdue University. He believes in translating the cutting-edge biology knowledge into tools to explore and reverse the epigenetic toxicity effects of environmental toxicants. He also works on targeted drug delivery with nanoparticles. Yi also has passion in teaching the next generation of bioengineers. Photochemical fate of quinone outside inhibitor fungicides
Issue Date:2021-04-27
Genre:Presentation / Lecture / Speech
Conference Paper / Presentation
Date Available in IDEALS:2021-04-26

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