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Title:Characterization of indigestible fiber in diets based on corn and soybean meal or corn, soybean meal, and distiller dried grains with solubles and in feces from pigs fed these diets
Author(s):Lancheros Castaneda, Jeimmy Paola
Advisor(s):Stein, Hans
Contributor(s):Cattai de Godoy, Maria; Fahey, Jr, George C.
Department / Program:Animal Sciences
Discipline:Animal Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:M.S.
Genre:Thesis
Subject(s):Arabinoxylan, enzymes, fiber, non-star polysaccharides, pig
Abstract:Three experiments were conducted to determine concentrations of non-starch polysaccharides, total dietary fiber and phenolic acids in a diet based on corn and soybean meal (SBM), and in a diet based on corn, SBM, and distiller dried grains with solubles (DDGS), as well as in feces from pigs fed these diets. The in-vitro disappearance of DM without and with inclusion of exogenous enzymes in both diets was also determined. In Exp. 1, total NSP and dietary fiber in diets based on corn and SBM or corn, SBM, and DDGS and in fecal samples from pigs fed these diets were quantified. The hypothesis that the concentration of NSP in the diet based on corn, SBM, and DDGS, and in feces from pigs fed this diet is greater than in the corn and SBM diet and in feces from pigs fed this diet, was tested. Concentration of total NSP in the diet containing corn, SBM, and DDGS was greater than in the diet based on corn and SBM. In contrast, the concentration of total NSP in the feces from pigs fed these diets was not different. The concentration of xylose, arabinose, and cellulose was predominant in both diets and feces from the pigs fed both diets. However, the digestibility of total NSP in the diet based on corn and SBM was greater than the digestibility from the diet based on corn, SBM, and DDGS. In Exp. 2, the ferulic and coumaric acid concentration in a diet based on corn and SBM and in a diet based on corn, SBM, and DDGS as well as in feces from pigs fed these diets was quantified. The hypothesis that the concentration of coumaric acid and ferulic acid is greater in the corn, SBM, and DDGS diet than in the corn and SBM diet, as well as in feces from pigs fed the corn, SBM, and DDGS diet compared with feces from pigs fed the corn and SBM diet was tested. The diet based on corn, SBM, and DDGS contained approximately 3 times as much coumaric acid and ferulic acid than the diet based on corn and SBM. Analysis of coumaric acid and ferulic acid in feces from pigs fed both diets indicated that only around one third of the bound phenolic acids are fermented in the intestinal tract of pigs indicating that coumaric and ferulic acids are major barriers to fermentation of arabinoxylans in corn fiber. In Exp. 3, the disappearance of DM in the indigestible fraction of a diet based on corn and SBM and in a diet based on corn, SBM, and DDGS was determined. Both diets were digested without inclusion of enzymes, with inclusion of four exogenous enzymes: arabinofuranosidase, feruloyl esterase, endo-xylanase, and cellobiohydrolase; and with 10 different combinations of these enzymes. The hypothesis that inclusion of xylanase by itself or in combination with cellobiohydrolase, arabinofuranosidase, or feruloyl esterase increases the digestibility of DM in the two diets was tested. Endo-xylanase may increase hydrolysis of arabinoxylan in corn and SBM diets and in corn, SBM, and DDGS diets when the enzyme is included in the diets, contributing to improved ATTD of DM. Inclusion of feruloyl esterase, cellobiohydrolase, and arabinofuranosidases individually or in combinations did not improve the ATTD of DM.
Issue Date:2021-01-05
Type:Thesis
URI:http://hdl.handle.net/2142/110405
Rights Information:Copyright 2021 Jeimmy Paola Lancheros Castaneda
Date Available in IDEALS:2021-09-17
Date Deposited:2021-05


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