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Title:Modulation of hippocampal synaptic transmission by the peptide diazepam binding inhibitor and optogenetic manipulation of astrocytes
Author(s):Courtney, Connor Duke
Director of Research:Christian-Hinman, Catherine A
Doctoral Committee Chair(s):Christian-Hinman, Catherine A
Doctoral Committee Member(s):Rhodes, Justin S; Sweedler, Jonathan V; Tsai, Nien-Pei
Department / Program:Neuroscience Program
Discipline:Neuroscience
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):diazepam binding inhibitor
astrocyte
synaptic transmission
hippocampus
Abstract:Hippocampal synaptic transmission is an intricate and flexible entity that can be modulated, both directly and indirectly, by a variety of different signaling molecules and cell types. The peptide diazepam binding inhibitor (DBI) has complex physiological functions and is capable of both enhancing and attenuating inhibitory synaptic transmission in different brain regions. However, whether DBI modulates hippocampal synaptic transmission or hippocampal-dependent behaviors has not previously been studied. In addition, astrocytes are increasingly recognized as dynamic regulators of excitatory synaptic transmission. However, the extent to which astrocytes modulate inhibitory transmission remains unknown. The overall goal of this dissertation is to investigate the degree to which hippocampal synaptic transmission is modulated by both DBI and by optogenetic stimulation of local astrocytes. Chapter 2 investigates the role of DBI in modulating hippocampal synaptic inhibition using a constitutive DBI knockout animal. Chapter 3 continues to use the DBI knockout animals to examine the role of DBI in regulating both hippocampal-dependent and hippocampal-independent behavior. Chapter 4 utilizes a novel astrocyte-specific Gq-coupled optogenetic construct (Optoα1AR) to probe astrocytic modulation of hippocampal synaptic excitation and inhibition. The results here indicate that DBI differentially modulates hippocampal synaptic transmission in CA1 versus dentate gyrus, and plays a notable role in spatial navigational memory. Furthermore, optogenetic stimulation of Optoα1AR in astrocytes modulates both hippocampal synaptic excitation and inhibition. However, this modulation depends on the characteristics of the optical stimulation paradigm. Taken together, these studies provide new evidence of DBI-dependent modulation of hippocampal synaptic inhibition and behavior, and provide a new optogenetic tool to study astrocytic regulation of synaptic transmission.
Issue Date:2021-04-21
Type:Thesis
URI:http://hdl.handle.net/2142/110530
Rights Information:Copyright 2021 Connor Courtney
Date Available in IDEALS:2021-09-17
Date Deposited:2021-05


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