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Title:Investigating the size effect of nanoparticles in Click Chemistry mediated cancer targeting
Author(s):Wang, Ying
Advisor(s):Cheng, Jianjun
Department / Program:Materials Science & Engineerng
Discipline:Materials Science & Engr
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Silica Nanoparticles, Cancer targeting, metabolic engineering
Abstract:Size is one of the most important properties of nanoparticles (NPs) that affects their biodistribution, accumulation and retention in the tumour tissue. The effect of NP size on tumour targeting has been studied in various contexts, with or without a targeting mechanism incorporated. Herein, we report our investigation of the size effect of NPs in Click Chemistry mediated cancer targeting. Realized by anchoring NPs onto the cell surface via metabolic glycoengineering for the cell surface placement of azido group followed by Click Chemistry mediated covalent reaction between the NP and the cell, we demonstrate that the combination effect of deeper penetration of smaller-size NP coupled with reduced clearance presumably due to the Click reaction results in its higher accumulation in the tumour tissue. Specifically, we firstly used metabolic glycoengineering to introduce azido groups to the cell surface, and then investigated the accumulation profiles of dibenzocyclooctyne conjugated nanoparticles bearing different sizes (20, 50 and 200 nm). The 20-nm silica nanoconjugates (NCs) were found to have the highest accumulation in the tumour tissue both ex vivo and in vivo. This work provides further insights into the design of nanomedicine for cancer targeting when the targeting is mediated by a non-conventional, covalent-chemistry-mediated approach.
Issue Date:2021-04-28
Rights Information:Copyright 2021 Ying Wang
Date Available in IDEALS:2021-09-17
Date Deposited:2021-05

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