Files in this item

FilesDescriptionFormat

application/pdf

application/pdfpone.0000833.pdf (396kB)
(no description provided)PDF

Description

Title:Differential Requirements for MCM Proteins in DNA Replication
Author(s):Crevel, Gilles; Hashimoto, Reina; Vass, Sharron; Sherkow, Jacob S.; Yamaguchi, Masamitsu; Heck, Margarete M.S.; Cotterill, Sue
Subject(s):DNA replication
MCM
drosophila
Abstract:The MCM2-7 proteins are crucial components of the pre replication complex (preRC) in eukaryotes. Since they are significantly more abundant than other preRC components, we were interested in determining whether the entire cellular content was necessary for DNA replication in vivo. We performed a systematic depletion of the MCM proteins in Drosophila S2 cells using dsRNA-interference. Reducing MCM2-6 levels by >95–99% had no significant effect on cell cycle distribution or viability. Depletion of MCM7 however caused an S-phase arrest. MCM2-7 depletion produced no change in the number of replication forks as measured by PCNA loading. We also depleted MCM8. This caused a 30% reduction in fork number, but no significant effect on cell cycle distribution or viability. No additive effects were observed by co-depleting MCM8 and MCM5. These studies suggest that, in agreement with what has previously been observed for Xenopus in vitro, not all of the cellular content of MCM2-6 proteins is needed for normal cell cycling. They also reveal an unexpected unique role for MCM7. Finally they suggest that MCM8 has a role in DNA replication in S2 cells.
Issue Date:2007-09-05
Publisher:PLoS One
Citation Info:Gilles Crevel et al., Differential Requirements for MCM Proteins in DNA Replication, 2 PLOS One 833 (2007)
Genre:Article
Type:Text
Language:English
URI:http://hdl.handle.net/2142/111006
DOI:10.1371/journal.pone.0000833
Date Available in IDEALS:2021-09-24


This item appears in the following Collection(s)

  • Illinois Research and Scholarship
    This is the default collection for all research and scholarship developed by faculty, staff, or students at the University of Illinois at Urbana-Champaign

Item Statistics