High density lipoproteins (HDL) can be fractionated by immunoaffinity chromatography into two main subclasses: a fraction which contains apolipoprotein (apo) AI and apoAII, and a fraction which contains apoAI but not apoAII. ...

LCAT glycosylation mutants N84Q and N384Q were examined to determine the effects of deleting individual glycan chains. Purified N84Q LCAT did not possess measurable enzymatic activity or interfacial binding affinity for ...

To separate interfacial binding from the catalytic reactions of LCAT, the first investigation of its reaction with water soluble substrates was performed to characterize the molecular specificity of the active site as well ...

The cDNA coding the human proapoA-I was cloned into an Escherichia coli vector, overexpressed and purified to 99% homogeneity and characterized together with apoA-I purified from human plasma. SDS-PAGE, mass spectrometry ...

Based on this study and previous results, a model of lipid-free proapoAI was constructed. This model takes into account the elongated shape of proapoAI, indicates that the N- and C-terminal extremes of proapoAI are compact, ...

Limited proteolysis was used to study the domain structure and to produce a large N-terminal fragment of human apolipoprotein A-I (apoA-I). Digestion of reconstituted high density lipoprotein (rHDL) prepared with apoA-I ...

High density lipoprotein (HDL) is believed to be involved in reverse cholesterol transport. HDL consists primarily of phospholipids, cholesterol, cholesterol esters, and apolipoprotein A-I (apo A-I). However, HDL is extremely ...