Browse Dept. of Biochemistry by Contributor "Kranz, David M."

• (1998)
In chapter two, the ability of CTL 2C to recognize thiol-modified peptide variants is analyzed. A method for the synthesis and screening of thiol-modified peptides that are recognized by T cells is presented. Chapter three ...

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• (2008)
Finally, in chapter 5, fluorescence spectroscopy was used in stability and binding studies of scTCRs. Urea denaturation analysis was used to show that scTCRs engineered in the yeast display system have similar stabilities ...

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• (1995)
The T cell receptor (TCR) is a cell surface protein that specifically binds to a foreign antigen on the surface of another cell. The TCR is made up of two disulfide-linked chains (called $\alpha$ and $\beta$) each containing ...

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• (1993)
Cytotoxic T lymphocytes (CTL) recognize and lyse cells presenting viral or tumor-associated antigens associated with class I major histocompatibility complex proteins. Recognition occurs between the T cell receptor (TCR) ...

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• (1997)
To begin to examine the 2C TCR residues which provide a significant thermodynamic contribution toward the binding energy of a variety of ligands, including monoclonal antibodies (mAb), superantigens, and peptide/MHC ligands, ...

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• (2002)
Chapter Four addresses the physiological T cell responses that can be elicited by peptide/MHC recognition. We demonstrated that the endogenously derived peptide, dEV8, functioned as both an agonist and an antagonist of T ...

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• (2012-06-27)
The essential family of aminoacyl-tRNA synthetase (AARS) enzymes catalyzes the attachment of an amino acid to its cognate tRNA during ribosome-based translation of mRNA. Leucyl-tRNA synthetase (LeuRS) ensures fidelity in ...

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• (1996)
Cancer is the one of the leading causes of death in the United States, and will affect one in three people in their lifetime. In addition to existing treatments, immunotherapy is emerging as a potential fourth tier of ...

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• (2002)
In Chapter 6, the role of TCR affinity in T cell activation was studied using CD8-negative T cells, as well as T cells expressing the co-receptor CD8. The peptide sensitivity of T cells that expressed the highest-affinity ...

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• (2015-01-21)
All nucleated cells display a sampling of their protein contents in the form of short 9-10 amino acid peptides bound to a product of the major histocompatibility complex (MHC) on the cell surface. This class of MHC-restricted ...

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• (2016-07-22)
The T cell receptor (TCR) is an heterodimer that binds to a short peptide bound to a product of the major histocompatibility complex (MHC). The chains each contain variable (V) and constant (C) region domains, followed ...

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• (1997)
In Chapter Five, a yeast display system was used to show that a T cell receptor ligand (scFv-KJ16) expressed on the surface of yeast was capable of inducing T cell activation. These findings suggest that the yeast display ...

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• (2011-01-21)
The alpha-beta T cell receptor (TCR) is responsible for mediating T cell recognition of self and non-self tissues, through recognition between a complex of a peptide and a product of the major histocompatibility complex ...

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• (2015-05-14)
Gram negative and positive bacteria have evolved toxins to aid in their ability to colonize host organisms. Some gram-positive bacteria produce exotoxins called superantigens that hyper-stimulate the immune system by ...

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• (2004)
Proteins encoded by the major histocompatability complex (MHC) were initially discovered based on their role in skin graft rejections. Years later, the physiological function of MHC proteins, as key players in the immune ...

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• (2015-04-21)
Staphylococcus aureus and group A Streptococcus express a family of exotoxins including staphylococcal enterotoxins A, B, C (SEA, SEB, SEC), toxic shock syndrome toxin-1 (TSST-1) and streptococcal pyrogenic exotoxins (SpeA, ...

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• (2007)
The overall goal of developing new engineering strategies to allow for the isolation of high affinity mutants of many different TCRs was extended to methods for affinity maturation in Chapter 4. Previous affinity-based ...

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• (2006)
Superantigens (SAgs) are potent pathogenic agents. Produced by bacteria and viruses, these proteins target a subset of white blood cells, namely T cells, which are responsible for the cell-mediated responses of the immune ...

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• (2011-01-14)
The focus of this research is the role of conformational flexibility in catalysis by a TIM-barrel enzyme in pyrimidine biosynthesis, orotidine 5’-monophosphate decarboxylase (OMPDC). OMPDC catalyzes the decarboxylation ...

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• (1995)
T cells are able to respond to foreign antigens by means of a diverse repertoire of antigen-specific receptors (T cell receptors or TCRs). There are two classes of TCRs ($\alpha\beta$ and $\gamma\delta$) that are found on ...

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