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Browse Dept. of Biochemistry by Contributor "Martinis, Susan A."
Now showing items 1-20 of 26
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(2009)The canonical genetic code has potentiel to be expanded by developing orthogonal aaRS:tRNA systems. Tools to generate an orthogonal LeuRSaRNA Leu pair were developed by using a two plasmid system in an E. coli strain ...
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(2009)The tRNA synthetases catalyze aminoacylation of transfer RNA (tRNA) with specific amino acids. Accurate aminoacylation is critical to the fidelity of protein synthesis. Leucyl-tRNA synthetase (LeuRS) can misactivate a broad ...
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(2010)Human cytoplasmic LeuRS (hscLeuRS) is typically found in a macromolecular complex containing at least eight other proteins. In order to study this enzyme, hscLeuRS was expressed independent of the complex in Escherichia ...
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(2007)Because they are essential to protein synthesis, aaRSs offer a promising target for the development of novel drugs. Leucyl-adenylate analogs were tested for inhibition activity against LeuRS from different sources. These ...
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(2010-05-14)Aminoacyl-tRNA synthetases (aaRSs) are ancient enzymes that charge tRNA with its cognate amino acid. In order to maintain fidelity during protein synthesis, editing mechanisms ensure that tRNAs are accurately charged. ...
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(2008)The accurate covalent linkage of amino acid to tRNA for protein synthesis is catalyzed by a family of aminoacyl-tRNA synthetases (aaRS). Some aaRSs have the potential to make mistakes during aminoacylation due to the nature ...
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(2007)We tested several mutations in yeast mitochondrial LeuRS that altered the post-transfer editing function of LeuRSs from other origins. Our results show that yeast mitochondrial LeuRS has maintained a competent editing ...
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(2012-06-27)The essential family of aminoacyl-tRNA synthetase (AARS) enzymes catalyzes the attachment of an amino acid to its cognate tRNA during ribosome-based translation of mRNA. Leucyl-tRNA synthetase (LeuRS) ensures fidelity in ...
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(2013-05-28)Information at the protein level is essential to understand the functioning of a biological system. The goal of proteomics is to provide such information as protein identities, post-translational modifications (PTMs) on ...
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(2010-01-06)In the genomic era, advanced sequencing techniques have enabled an exponential growth of the protein sequence databases. Although the abundance of genomic sequences is valuable, the correctness of the gene annotations ...
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(2008)Individual glycine residues, when mutated to proline, misaminoacylate tRNALeu in spite of retaining efficient amino acid editing activities. It is possible that these mutant LeuRSs may have impaired the translocation of ...
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing (2013-08-22)In the post-genome era, one of the most important topics of research is to edit or program genomic sequences and to generate desired phenotypes. Although virus-based strategies have long been developed to for efficient ...
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(2015-04-21)Staphylococcus aureus and group A Streptococcus express a family of exotoxins including staphylococcal enterotoxins A, B, C (SEA, SEB, SEC), toxic shock syndrome toxin-1 (TSST-1) and streptococcal pyrogenic exotoxins (SpeA, ...
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(2014-01-16)The ribosome translates genomic information into structural and catalytic protein molecules. In addition to its role in protein synthesis, the ribosome also produces small non-catalytic peptides that exert antimicrobial ...
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From yeast to animals: A small molecule restores iron mobilization, absorption and hemoglobinization (2019-03-15)In the classic paradigm of pharmacology, small molecules bind to and modulate dysfunctional or overactive proteins. However, in cases where a protein is missing, this strategy is no longer viable. Disorders of iron metabolism ...
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(2013-02-03)Nature has given nucleic acids irreplaceable roles for all living creatures. It plays important roles in genetic information storage and transfer, as well as regulating various aspects of biology. The discovery of in vitro ...
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(2015-01-21)Since the discovery of nucleic acid enzymes and aptamers that can perform functional roles other than storing genetic information; a new paradigm in nucleic acid chemistry has been opened. In 1990 the first artificially ...
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(2017-02-10)Nature has developed the use of proteins and RNA as enzymes, while DNA is used for the storage and transfer of genetic information. Proteins and RNA are biopolymers that can fold into specific secondary and tertiary ...
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(2013-08-22)Pre-mRNA splicing in plants is mechanistically the same as splicing in other eukaryotes, but its mode of intron recognition is unique. Whereas in animals a pyrimidine-tract contributes to the definition of exons, in plants, ...
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(2015-02-25)CYP19A1 or aromatase, is a human steroidogenic P450 important for estrogen biosynthesis in humans. Over activation of aromatase results in malignancies of the breast tissue, especially in post menopausal women. In fact, ...
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Now showing items 1-20 of 26