Mathematical and experimental tools for the measurement and assessment of association and dissociation kinetics in anti-fluorescyl antibodies were developed in these studies. An analytic procedure for the characterization ...

Genetic tools were developed to study gene regulation in Rhodopseudomonas sphaeroides. In initial studies expression of the transposable lac operon, Tn951, was examined. Very low levels of (beta)-galactosidase were observed ...

PR4 is a bacteriophage that contains lipid bilayer membrane within its protein capsid. The hosts include Escherichia coli and Salmonella typhimurium.

The goal of this study was to understand the mechanism by which manganese protects cells against reactive oxygen species, particularly H2O2. We showed while manganese transport mutants, mntH, have no growth defect, mntH ...

The goal of this study was to determine the mechanism of copper toxicity. Copper is toxic to both eukaryotes and prokaryotes and is the causative agent of Indian childhood cirrhosis, Tyrolean infantile cirrhosis, and Wilson ...

Organelle transport in eukaryotic cells is a process essential to many cellular functions. Understanding the mechanisms that drive and regulate these movements is, therefore, an important goal for cell biology. We have ...

Base pairing small regulatory RNAs (sRNAs) are important post-transcriptional regulators of gene expression in bacteria. These sRNAs deploy novel mechanisms to regulate mRNA targets leading to various physiological outcomes ...

An Escherichia coli strain that cannot scavenge hydrogen peroxide has been used to identify the cell processes that are most sensitive to this oxidant. Low micromolar concentrations of H2O2 completely blocked the biosynthesis ...