Bacteroides spp. are obligately anaerobic Gram-negative bacilli that reside in high numbers in the colons of humans and animals. It has been estimated that Bacteroides spp. constitute as high as 30% of all colonic isolates. ...

The formation of the embryonic lens is a classic system for the study of tissue interactions, termed inductive interactions. Chapter 1 will present an overview of lens formation, focusing on the inductive interactions ...

The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated ...

A Tris-dependent transformation system was developed for R. sphaeroides. Washing the cells with 0.5M Tris-buffer, pH 7.2, at 0(DEGREES)C prior to exposure to DNA was found to be necessary for inducing a competent state for ...

To study the mechanism of transcription termination at a rho-independent terminator, homoduplex and heteroduplex templates that contained mutations in the G + C-rich region of dyad symmetry in the Escherichia coli thr ...

Conjugation assays determined that the bacteria mate in a cell density dependent manner, known as quorum-sensing. Analysis of transfer frequencies using a transfer-constitutive mutant of pTiC58 showed that quorum sensing ...

New Zealand BW mice spontaneously develop lymphocytic disorders and express autoantibodies in association with an autoimmune disease similar to human SLE. A comparative monoclonal analysis of spontaneous and experimentally-induced ...

To study Salmonella pathogenesis we used the IVET ( in vivo expression technology) system to select S. typhimurium genes that are specifically induced in the host during infection. This unique IVET selection was done in ...

Mutations within the lambda attachment (att) site were constructed by oligonucleotide-directed site-specific mutagenesis and analysed for their effects on site-specific recombination. The phenotype of mutations within the ...

NBU1 is a 10.3 kbp mobilizable transposon that is normally integrated in the chromosome of its Bacteroides host. Bacteroides are the predominant member of the human intestinal tract microflora, and are opportunistic ...