CTnDOT is a conjugative transposon (CTn) that is found in many Bacteroides strains. Transfer of CTnDOT is stimulated 100--1000 fold if the cells are exposed to tetracycline (Tc). An operon that contains a Tc resistance ...

I constructed the E. coli-Bacteroides shuttle vector, pVAL-1. This was the first in a series of new cloning vehicles that made it easier to clone Bacteroides DNA in E. coli and mobilize it from E. coli to Bacteroides species.

A tetracycline resistance ($\rm \sp{*}Tc\sp{r}$) gene that was originally found on two Bactetoides transposons, Tn4351 and Tn4400, confers resistance on Escherichia coli, but only when the cells are grown aerobically. I ...

In general, very little is known about the way in which Bacteroides conjugative transposons integrate into their chromosomal target sites, or what the nature of these sites are. In the past, this question has been very ...

One of the growing interests in bacterial fatty acid biosynthesis is due to the fact that an increasing number of both synthetic and natural antibacterial compounds specifically target fatty acid biosynthesis enzymes. ...

This dissertation presents the first detailed study of the conjugal transfer genes found on a conjugative transposon. The complete sequence of a functional transfer region is presented and comparative analysis is made ...

In this study, we showed that expression of the exc operon is regulated by tetracycline and mediated by the regulatory proteins, RteA, RteB, and RteC. Tetracycline induces the expression of rteA and rteB, genes in the ...

Polysaccharides are a major source of carbon and energy for the bacteria that normally reside in the human colon. The finding that some of the major colonic species such as Bacteroides thetaiotaomicron can utilize host-derived ...

In this study, the minimal starch utilization system was used to study interactions among SusC, SusD, and SusG. To examine interactions between SusE and SusF, wild type and other mutants were used. For this study, several ...

My thesis details studies of the extrachromosomal elements of Lactobacillus acidophilus strain 100-33. Strain 100-33 has antibiotic resistance to macrolide, lincosamide, and streptogramin B-type antibiotics (MLS$\sp{\rm ...