IDEALS Home University of Illinois at Urbana-Champaign logo The Alma Mater The Main Quad

Effects of sodium hyaluronate and triamcinolone acetonide on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1

Show full item record

Bookmark or cite this item: http://hdl.handle.net/2142/16488

Files in this item

File Description Format
PDF 1_Schaefer_Elysia.pdf (856KB) (no description provided) PDF
Title: Effects of sodium hyaluronate and triamcinolone acetonide on proteoglycan metabolism in equine articular chondrocytes treated with interleukin-1
Author(s): Schaefer, Elysia C.
Advisor(s): Stewart, Allison A.
Department / Program: Vet Clinical Medicine
Discipline: VMS-Veterinary Clinical Medcne
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: M.S.
Genre: Thesis
Subject(s): equine chondrocytes osteoarthritis triamcinolone hyaluronic acid
Abstract: The objective of this study is to determine if the effects of a high molecular weight sodium hyaluronate (HA) alone or in combination with triamcinolone acetate (TA) can mitigate chondrocyte proteoglycan catabolism caused by interleukin-1 (IL-1) administration. Chondrocytes were collected from fetlock joints of ten horses euthanized for reasons unrelated to joint disease. Chondrocyte pellets were treated with media (negative control); media containing IL-1 only (positive control); or media containing IL-1 with HA only (0.5 or 2.0 mg/mL), TA only (0.06 or 0.6 mg/mL), or HA (0.5 or 2.0 mg/mL) and TA (0.06 or 0.6 mg/mL) in combination. Chondrocyte pellets were assayed for newly synthesized GAG, total GAG content, total DNA content, and mRNA levels of collagen type II, aggrecan, and COX-2. The high concentration of HA (2.0 mg/mL) increased GAG synthesis while the high concentration of TA (0.6 mg/mL) decreased loss of GAG into the media. Both the high concentration of HA and TA increased the total GAG content within the pellet. There was no change in pellet DNA content with either treatment. TA reduced COX-2 mRNA levels as well as aggrecan and collagen type II expression. Treatment with HA had no effect on mRNA levels of COX-2, aggrecan or collagen type II. These results indicate that the high concentration of HA or TA alone or in combination will mitigate effects of IL-1 administration on proteoglycan catabolism of equine articular chondrocytes.
Issue Date: 2010-06-22
URI: http://hdl.handle.net/2142/16488
Rights Information: Copyright 2010 Elysia Schaefer
Date Available in IDEALS: 2010-06-22
Date Deposited: May 2010
 

This item appears in the following Collection(s)

Show full item record

Item Statistics

  • Total Downloads: 688
  • Downloads this Month: 12
  • Downloads Today: 1

Browse

My Account

Information

Access Key