IDEALS Home University of Illinois at Urbana-Champaign logo The Alma Mater The Main Quad

Characterization and modeling of cell activation upon TGF-β1 stimulation in a 3-D culture system

Show full item record

Bookmark or cite this item: http://hdl.handle.net/2142/16963

Files in this item

File Description Format
PDF 3_Xu_Jing.pdf (2MB) (no description provided) PDF
Title: Characterization and modeling of cell activation upon TGF-β1 stimulation in a 3-D culture system
Author(s): Xu, Jing
Advisor(s): Insana, Michael F.
Department / Program: Bioengineering
Discipline: Bioengineering
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: M.S.
Genre: Thesis
Subject(s): myofibroblasts transforming growth factor-beta1 alpha-smooth muscle actin diffusion confocal microscopy imaging immunofluorescence staining
Abstract: In this work, we explored a thick in vitro 3-D cell culture model with layered structure and applied transforming growth factor-beta1 (TGF-β1) on the top of the 3-D culture to induce the conversion from normal fibroblasts to myofibroblasts and expression of a marker protein, alpha-smooth muscle actin (α-SMA). The cell response with TGF-β1 stimulation was captured with immunofluorescence staining (IF) and confocal microscopy imaging of α-SMA in each cell embedded layer. Our results revealed the spatial-temporal profiles of the conversion from normal fibroblasts to myofibroblasts in 3-D culture upon TGF-β1stimulation. The conversion starting time, speed and saturation plateau are closely related to TGF-β1 concentration. Based on these experimental results, we successfully developed a mathematical model, which incorporates diffusion of growth factors and cell activation responses to describe the dynamic cell response to growth factors across the 3-D culture depth and predict the spatial-temporal profile of the fibroblast conversion within the culture. In summary, we present here a handy experimental and computational tool for researches on tumor signaling pathways, and provide some guidance for studies on diffusion of protein factors or drugs in 3-D tissue environment.
Issue Date: 2010-08-31
URI: http://hdl.handle.net/2142/16963
Rights Information: Copyright 2010 Jing Xu
Date Available in IDEALS: 2010-08-31
2012-09-07
Date Deposited: 2010-08
 

This item appears in the following Collection(s)

Show full item record

Item Statistics

  • Total Downloads: 58
  • Downloads this Month: 0
  • Downloads Today: 0

Browse

My Account

Information

Access Key