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High-throughput detection of protein—protein and protein—DNA interactions via photonic crystal biosensors: applications to apoptosis inducing factor

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Title: High-throughput detection of protein—protein and protein—DNA interactions via photonic crystal biosensors: applications to apoptosis inducing factor
Author(s): Heeres, James T.
Director of Research: Hergenrother, Paul J.
Doctoral Committee Chair(s): Hergenrother, Paul J.
Doctoral Committee Member(s): Cunningham, Brian T.; Shapiro, David J.; Morrissey, James H.
Department / Program: Biochemistry
Discipline: Biochemistry
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: Ph.D.
Genre: Dissertation
Subject(s): Photonic Crystals Apoptosis Inducing Factor apoptosis inducing factor (AIF) Protein-DNA Interactions Protein-Protein Interactions
Abstract: High-throughput screening (HTS) has played an integral role in the discovery of lead molecules that eventually are transformed into the therapies of tomorrow. Contributing towards the success of any screening campaign is the target choice, chemical library size and diversity, as well as the technology utilized in screening. Photonic crystal (PC) biosensors are a relatively new technology whose potential is only beginning to be realized. PC biosensors are a “labelfree” technology compatible with standard high-throughput techniques. Macromolecular interactions including protein—small molecule, protein—nucleic acid, protein—protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein—nucleic acid interaction and a simplified protein— protein interaction platform. The protein—nucleic acid interaction targeted is the apoptosis inducing factor (AIF)—DNA interaction, which is critical for the mediation of caspaseindependent cell death in neurodegenerative disease and stroke. Protein—protein interactions were targeted by modifying the PC biosensor surface for detection of the His6 tag, which is widely employed in protein purification.
Issue Date: 2010-08-31
URI: http://hdl.handle.net/2142/17028
Rights Information: Copyright 2010 James T. Heeres
Date Available in IDEALS: 2010-08-31
2012-09-07
Date Deposited: 2010-08
 

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