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Title:Engineered Vesicles for Perchlorate Degradation
Author(s):Poust, Sean K.
Advisor(s):Zilles, Julie L.
Department / Program:Civil & Environmental Eng
Discipline:Environ Engr in Civil Engr
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Polymer vesicles
Perchlorate treatment
Synthetic biology
Water treatment
Abstract:This study demonstrates the use of engineered vesicles to reduce perchlorate. Specifically, cell-free extracts containing perchlorate reductase and chlorite dismutase enzymes were encapsulated in a triblock copolymer vesicle functionalized with the outer membrane porin OmpF. The porin allows for perchlorate transport into the vesicles, inside which the encapsulated enzymes transform perchlorate to chloride. Perchlorate reduction was quantified using a methyl viologen colorimetric technique. The vesicle solutions had perchlorate-reducing activities ranging from 35-45 units per liter. This work shows that vesicles can provide a mechanism to utilize environmentally-relevant biological enzymes. When incorporated into a vesicle, the enzymes could be used outside of environmental conditions where they would normally be expressed by natural bacteria.
Issue Date:2010-08-31
Rights Information:Copyright 2010 Sean K. Poust
Date Available in IDEALS:2010-08-31
Date Deposited:2010-08

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