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Human FRG1 is an Actin-Bundling and a RNA-Associated Protein with Distinct Subcellular Localizations

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Title: Human FRG1 is an Actin-Bundling and a RNA-Associated Protein with Distinct Subcellular Localizations
Author(s): Sun, Chia-Yun
Director of Research: Jones, Peter L.
Doctoral Committee Chair(s): Bellini, Michel
Doctoral Committee Member(s): Jones, Peter L.; Brieher, William M.; Ceman, Stephanie S.; Nardulli, Ann M.
Department / Program: Cell & Developmental Biology
Discipline: Cell and Developmental Biology
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: Ph.D.
Genre: Dissertation
Subject(s): FSHD region gene 1 (FRG1) RNA biogenesis mRNA transport Actin Z-disc Myogenesis Facioscapulohumeral muscular dystrophy (FSHD) Muscular dystrophy
Abstract: Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is critical for development of the vertebrate musculature and vasculature, however its precise molecular function is unknown. Because of its location 125 kb proximal to the FSHD1A lesion, a deletion in a subtelomeric macrosatellite DNA repeat array, it has been considered a candidate for mediating FSHD pathophysiology. This study investigates FRG1’s function to provide insight into FRG1’s role in vertebrate muscle development. First, we focus on the subcellular localization of FRG1 and identified FRG1 as a dynamic nuclear, cytoplamic, and sarcomeric protein. During myoblast differentiation, FRG1’s subcellular distribution changed dramatically with FRG1 eventually associating with the matured Z-discs. The Z-disc localization is confirmed in mouse myofibers, suggesting FRG1 may have a muscle-specific function involved in sarcomere maintenance or signaling. The nuclear fraction of the endogenous FRG1 is localized in nucleoli, Cajal bodies, and actively transcribed chromatin associated with nascent RNA transcripts, supporting a function in RNA biogenesis. Furthermore, we show that FRG1 interacts specifically with RNA in vitro, associates with mRNA in vivo, and directly interacts with the TAP mRNA export receptor. FRG1 also exists in a cytoplasmic pool that is dependent on an intact actin cytoskeleton for its localization and we demonstrate FRG1 itself is an actin binding protein. These data provide the first biochemical activities for human FRG1 and indicate that FRG1 dynamically shuttles between the nucleus and cytoplasm and is involved in aspects of RNA biogenesis, potentially including mRNA transport and localization.
Issue Date: 2011-01-21
URI: http://hdl.handle.net/2142/18617
Rights Information: Copyright 2010 Chia-Yun Sun
Date Available in IDEALS: 2011-01-21
2013-01-22
Date Deposited: 2010-12
 

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