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|Title:||Mutational analysis of integrase and excisionase proteins in bacteriophage lambda site-specific recombination system|
|Doctoral Committee Chair(s):||Gardner, Jeffrey F.|
|Doctoral Committee Member(s):||Gumport, Richard I.; Voss, Edward W., Jr.|
|Department / Program:||Microbiology|
|Degree Granting Institution:||University of Illinois at Urbana-Champaign|
|Abstract:||Second-site reversion analyses were performed on 21 recombination defective Int mutants. A second-site revertant P243L/E218K and a pseudorevertant E175K were isolated and further characterized using genetic and biochemical methods. The results showed that the second-site substitution E218K selectively enhances Int core-binding affinity and acts as a global suppressor. In addition, our genetic data suggest that position 218 is part of or near the putative Int core-binding domain.
Saturation mutagenesis was performed on the region of the xis gene that encodes carboxyl region of the Xis protein. Forty-five new C-terminal region mutants were isolated and their abilities to promote excision, to bind the DNA and to interact with Int and FIS were characterized in vivo and in vitro. In addition, the secondary structure of this region was predicted by computer modeling schemes. A model for the secondary structures of carboxyl region of Xis protein was developed by combining the mutagenesis information with computer-based analytic techniques. According to the model, residues between 57 to 65 are located in an $\alpha$-helix structure. The functional study of mutants within the helix suggests that one side of the helix (residues 57, 60, 63 and 64) interacts with Int. In addition, the identification of structurally stable mutants of Xis may be useful in studying its degradation pathway and in supplying protein for crystallographic studies.
|Rights Information:||Copyright 1995 Wu, Zhao|
|Date Available in IDEALS:||2011-05-07|
|Identifier in Online Catalog:||AAI9624540|