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Title:Effects of selenite on metabolic events during the proliferation of canine mammary tumor cells
Author(s):Hwang, Kyung Hee
Doctoral Committee Chair(s):Milner, J.A.
Department / Program:Nutritional Sciences
Discipline:Nutritional Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Molecular
Biology, Cell
Agriculture, Animal Culture and Nutrition
Abstract:Increased cytoplasmic and nuclear selenium retention in CMT-13 cells was correlated with cell growth inhibition caused by selenite supplementation. Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells increased. One of the antiproliferation effects of selenite on CMT-13 cells is accompanied by decreased rates of macromolecule synthesis, increased cellular macromolecule contents and increased cell size and multiple nuclei, indicating that enhanced cell fusion occurred. Selenite modulated the S and M phases during the cell cycle. Selenite increased the activity of the 114 KD protein kinase only during the S phase. Selenite treatment during the M phase resulted in a disappearance of a 53 KD kinase and the appearance of a 47 KD kinase. The cellular $\rm\sp{32}P$ incorporation into macromolecules increased when inhibition of cell growth was observed by supplementation of selenite. Selenite generally increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein phosphorylation may explain the ability of selenite to alter cell proliferation.
Issue Date:1994
Rights Information:Copyright 1994 Hwang, Kyung Hee
Date Available in IDEALS:2011-05-07
Identifier in Online Catalog:AAI9503220
OCLC Identifier:(UMI)AAI9503220

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