IDEALS Home University of Illinois at Urbana-Champaign logo The Alma Mater The Main Quad

Effects of selenite on metabolic events during the proliferation of canine mammary tumor cells

Show full item record

Bookmark or cite this item:

Files in this item

File Description Format
PDF 9503220.pdf (6MB) Restricted to U of Illinois (no description provided) PDF
Title: Effects of selenite on metabolic events during the proliferation of canine mammary tumor cells
Author(s): Hwang, Kyung Hee
Doctoral Committee Chair(s): Milner, J. A.
Department / Program: Nutritional Sciences
Discipline: Nutritional Sciences
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: Ph.D.
Genre: Dissertation
Subject(s): Biology, Molecular Biology, Cell Agriculture, Animal Culture and Nutrition
Abstract: Increased cytoplasmic and nuclear selenium retention in CMT-13 cells was correlated with cell growth inhibition caused by selenite supplementation. Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells increased. One of the antiproliferation effects of selenite on CMT-13 cells is accompanied by decreased rates of macromolecule synthesis, increased cellular macromolecule contents and increased cell size and multiple nuclei, indicating that enhanced cell fusion occurred. Selenite modulated the S and M phases during the cell cycle. Selenite increased the activity of the 114 KD protein kinase only during the S phase. Selenite treatment during the M phase resulted in a disappearance of a 53 KD kinase and the appearance of a 47 KD kinase. The cellular $\rm\sp{32}P$ incorporation into macromolecules increased when inhibition of cell growth was observed by supplementation of selenite. Selenite generally increased the phosphorylation of nuclear phosphoproteins in the S phase, especially the phosphorylation of a 46 KD protein. Selenite inhibited hyperphosphorylation of the 21, 62 and 108 KD proteins during the M phase. Results of this study suggest that perturbations in protein phosphorylation may explain the ability of selenite to alter cell proliferation.
Issue Date: 1994
Type: Text
Language: English
Rights Information: Copyright 1994 Hwang, Kyung Hee
Date Available in IDEALS: 2011-05-07
Identifier in Online Catalog: AAI9503220
OCLC Identifier: (UMI)AAI9503220

This item appears in the following Collection(s)

Show full item record

Item Statistics

  • Total Downloads: 0
  • Downloads this Month: 0
  • Downloads Today: 0


My Account


Access Key