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Quinol oxidation by the ubiquinol:cytochrome c(2) oxidoreductase in the chromatophores from Rhodobacter sphaeroides

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Title: Quinol oxidation by the ubiquinol:cytochrome c(2) oxidoreductase in the chromatophores from Rhodobacter sphaeroides
Author(s): Wang, Zhenggan
Doctoral Committee Chair(s): Crofts, Antony R.
Department / Program: Biophysics and Computational Biology
Discipline: Biophysics
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: Ph.D.
Genre: Dissertation
Subject(s): Biophysics, General
Abstract: The kinetics and thermodynamics of the high potential chain in the chromatophores from Rhodobacter sphaeroides have been reexamined. It was found that RC, cyt c$\sb2$ and c$\sb1$ after a flash reached a state not far from the expected ideal equilibrium under the conditions of our experiments.An extinction coefficient for cyt b$\sb{\rm H}$ was computed from comparison of oxidation kinetics of c cytochromes in the presence of antimycin and UHDBT. The effects of myxothiazol on the cyt c oxidation have also been discussed.Our observations of the temperature dependence of cyt b reduction suggested that quinone diffusion was more rapid than turnover of the bc$\sb1$ complex, and that the chemical step of quinol oxidation was the rate-limiting step in the photosynthetic electron transport of chromatophore system.Oxidation kinetic of quinol by bc$\sb1$ complex in chromatophores was simulated. A set of kinetic parameters were obtained from optimization, and compared with experimental results. A minimal model of quinol oxidation was proposed, and discussed under the context of the Q-cycle mechanism.
Issue Date: 1990
Type: Text
Language: English
URI: http://hdl.handle.net/2142/19323
Rights Information: Copyright 1990 Wang, Zhenggan
Date Available in IDEALS: 2011-05-07
Identifier in Online Catalog: AAI9026347
OCLC Identifier: (UMI)AAI9026347
 

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