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Title:Quinol oxidation by the ubiquinol:cytochrome c(2) oxidoreductase in the chromatophores from Rhodobacter sphaeroides
Author(s):Wang, Zhenggan
Doctoral Committee Chair(s):Crofts, Antony R.
Department / Program:Biophysics and Computational Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biophysics, General
Abstract:The kinetics and thermodynamics of the high potential chain in the chromatophores from Rhodobacter sphaeroides have been reexamined. It was found that RC, cyt c$\sb2$ and c$\sb1$ after a flash reached a state not far from the expected ideal equilibrium under the conditions of our experiments.
An extinction coefficient for cyt b$\sb{\rm H}$ was computed from comparison of oxidation kinetics of c cytochromes in the presence of antimycin and UHDBT. The effects of myxothiazol on the cyt c oxidation have also been discussed.
Our observations of the temperature dependence of cyt b reduction suggested that quinone diffusion was more rapid than turnover of the bc$\sb1$ complex, and that the chemical step of quinol oxidation was the rate-limiting step in the photosynthetic electron transport of chromatophore system.
Oxidation kinetic of quinol by bc$\sb1$ complex in chromatophores was simulated. A set of kinetic parameters were obtained from optimization, and compared with experimental results. A minimal model of quinol oxidation was proposed, and discussed under the context of the Q-cycle mechanism.
Issue Date:1990
Rights Information:Copyright 1990 Wang, Zhenggan
Date Available in IDEALS:2011-05-07
Identifier in Online Catalog:AAI9026347
OCLC Identifier:(UMI)AAI9026347

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