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Title:Localization of P450 aromatase in sperm and estrogen receptors in the epididymal region of the rooster
Author(s):Kwon, Seok
Doctoral Committee Chair(s):Bahr, Janice M.
Department / Program:Animal Sciences
Discipline:Animal Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Cell
Biology, Animal Physiology
Abstract:The epididymal region of the rooster (rete testis, efferent ductules, and epididymal duct) has an important role in the production of fertile sperm by controlling the luminal microenvironment required for sperm maturation. Even though androgen is the major hormone involved in regulating functions of the epididymal region, there are many other regulatory molecules in testicular fluid that appear to play a role. The presence of estrogen receptors (ER) in the male reproductive tract suggests that estrogen may be important for epididymal functions. This investigation was undertaken to test the hypothesis that estrogen has a role in the regulation of the male reproductive system of the rooster. Specific objectives of my dissertation were: (1) to determine if germ cells and epididymal sperm are sources of estrogen; (2) to determine if the epididymal region of the rooster is a target for estrogen; and (3) to determine if testicular steroids regulate the expression of ER in the epididymal region of the rooster. The major findings were: (1) adult rooster testicular germ cells and epididymal sperm contain active P450 aromatase (P450arom) which converts androgens to estrogens; (2) ER and ER mRNA are present in the epididymal region of the rooster, which suggests that this region is a target for estrogen; and (3) steady state levels of ER mRNA in the epididymal region of roosters was suppressed in the presence of reduced serum concentrations of testosterone. These data clearly demonstrate that rooster germ cells and epididymal sperm are sites for the synthesis of estrogen and the epididymal region of the rooster is a target for estrogen. Preliminary studies suggest that testicular steroids may regulate the expression of ER in the epididymal region of the rooster. The presence of estrogen as well as ER in the epididymal region suggests that estrogen might be involved in the regulation of epididymal functions in the rooster.
Issue Date:1996
Type:Text
Language:English
URI:http://hdl.handle.net/2142/19964
ISBN:9780591088137
Rights Information:Copyright 1996 Kwon, Seok
Date Available in IDEALS:2011-05-07
Identifier in Online Catalog:AAI9702570
OCLC Identifier:(UMI)AAI9702570


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