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|Title:||Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer|
|Doctoral Committee Chair(s):||Salyers, Abigail A.|
|Department / Program:||Microbiology|
|Degree Granting Institution:||University of Illinois at Urbana-Champaign|
|Abstract:||Physical mapping techniques were employed to construct both a physical and genetic maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this $\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\pm$ 95 and 914 $\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb.
The smallest, 42 kb, endogenous plasmid was observed to be self transmissible. As little as an 425 bp oriT-containing DNA fragment isolated from this plasmid was able to confer transfer, between R. sphaeroides strains, when it was cloned into an otherwise non-transmissible plasmid. The DNA sequence of this oriT containing fragment reveals an A-T rich region, several direct and inverted repeats, as well as putative IHF-binding sites.
Random insertion of oriT into the R. sphaeroides 2.4.1 genome generated Hfr-like strains which exhibit a gradient of marker transfer, and has further provided classical genetic evidence for the circularity as well as the presence of two distinct chromosomal linkage groups in this bacterium.
|Rights Information:||Copyright 1992 Suwanto, Antonius|
|Date Available in IDEALS:||2011-05-07|
|Identifier in Online Catalog:||AAI9215894|