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Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer
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http://hdl.handle.net/2142/20426
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| Title: | Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer |
| Author(s): | Suwanto, Antonius |
| Doctoral Committee Chair(s): | Salyers, Abigail A. |
| Department / Program: | Microbiology |
| Discipline: | Microbiology |
| Degree Granting Institution: | University of Illinois at Urbana-Champaign |
| Degree: | Ph.D. |
| Genre: | Dissertation |
| Subject(s): |
Biology, Molecular
Biology, Genetics Biology, Microbiology |
| Abstract: | Physical mapping techniques were employed to construct both a physical and genetic maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this $\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\pm$ 95 and 914 $\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb. The smallest, 42 kb, endogenous plasmid was observed to be self transmissible. As little as an 425 bp oriT-containing DNA fragment isolated from this plasmid was able to confer transfer, between R. sphaeroides strains, when it was cloned into an otherwise non-transmissible plasmid. The DNA sequence of this oriT containing fragment reveals an A-T rich region, several direct and inverted repeats, as well as putative IHF-binding sites. Random insertion of oriT into the R. sphaeroides 2.4.1 genome generated Hfr-like strains which exhibit a gradient of marker transfer, and has further provided classical genetic evidence for the circularity as well as the presence of two distinct chromosomal linkage groups in this bacterium. |
| Issue Date: | 1992 |
| Type: | Text |
| Language: | English |
| URI: | http://hdl.handle.net/2142/20426 |
| Rights Information: | Copyright 1992 Suwanto, Antonius |
| Date Available in IDEALS: | 2011-05-07 |
| Identifier in Online Catalog: | AAI9215894 |
| OCLC Identifier: | (UMI)AAI9215894 |
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Graduate Theses and Dissertations at Illinois
Graduate Theses and Dissertations at Illinois -
Dissertations - Microbiology
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