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Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer

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Title: Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer
Author(s): Suwanto, Antonius
Doctoral Committee Chair(s): Salyers, Abigail A.
Department / Program: Microbiology
Discipline: Microbiology
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: Ph.D.
Genre: Dissertation
Subject(s): Biology, Molecular Biology, Genetics Biology, Microbiology
Abstract: Physical mapping techniques were employed to construct both a physical and genetic maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this $\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\pm$ 95 and 914 $\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb.The smallest, 42 kb, endogenous plasmid was observed to be self transmissible. As little as an 425 bp oriT-containing DNA fragment isolated from this plasmid was able to confer transfer, between R. sphaeroides strains, when it was cloned into an otherwise non-transmissible plasmid. The DNA sequence of this oriT containing fragment reveals an A-T rich region, several direct and inverted repeats, as well as putative IHF-binding sites.Random insertion of oriT into the R. sphaeroides 2.4.1 genome generated Hfr-like strains which exhibit a gradient of marker transfer, and has further provided classical genetic evidence for the circularity as well as the presence of two distinct chromosomal linkage groups in this bacterium.
Issue Date: 1992
Type: Text
Language: English
URI: http://hdl.handle.net/2142/20426
Rights Information: Copyright 1992 Suwanto, Antonius
Date Available in IDEALS: 2011-05-07
Identifier in Online Catalog: AAI9215894
OCLC Identifier: (UMI)AAI9215894
 

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