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Title:The influence of the interaction of fluoride (F) and aluminum (Al) on bioavailability in bacterial and mammalian systems
Author(s):Ahn, Hye-Won
Doctoral Committee Chair(s):Jeffery, Elizabeth H.
Department / Program:Veterinary Biosciences
Discipline:Veterinary Biosciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Health Sciences, Toxicology
Health Sciences, Public Health
Environmental Sciences
Abstract:Fluoride and aluminum, which are known to form a strong complex, are both present in finished drinking water. The effect of F and Al on one another's tissue accumulation was determined using adult, male New-Zealand white rabbits. Fluoride accumulation in plasma, urine, incisors and tibia was increased as the F addition to the drinking water increased within groups receiving a single concentration of Al. In contrast, F accumulation in plasma, urine, incisors and tibia decreased as the Al concentration increased within groups receiving a single F concentration. In addition, Al levels of plasma and tibia were significantly increased by the addition of F to the drinking water, even in animals receiving no Al in their drinking water. Therefore, some of the toxicity associated with high F level might in fact be associated with Al or a combined form of F and Al.
Because in vivo studies have shown that F causes the co-accumulation of Al in bone, we determined the interactive effect of these two agents on bone turnover and accumulation in mouse calvarial culture. The combination of F and Al increases alkaline phosphatase activity in calvaria. However, the co-accumulation of Al in bone treated with F that we reported using in an in vivo system could not be confirmed using a calvaria culture system, due to high non-specific adsorption of Al.
Further, the effect of Al on F uptake and mutagenicity in Salmonella typhymurium TA98 has been determined and any effect of Al on Ames test results for F was also determined. Intracellular F was below the limit of detection (0.2 ppm). However, F was taken up from media, providing that Al was also present. Neither F, Al, nor Al:F was found to be mutagenic as assessed by a modification of the Ames standard plate incorporation assay. While F did not appear to cause mutations in the presence or absence of Al, it was concluded that the mutagenicity test, using TA98, is not suitable for the evaluation of F mutagenicity.
Issue Date:1994
Type:Text
Language:English
URI:http://hdl.handle.net/2142/20951
Rights Information:Copyright 1994 Ahn, Hye-Won
Date Available in IDEALS:2011-05-07
Identifier in Online Catalog:AAI9503125
OCLC Identifier:(UMI)AAI9503125


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