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A stable genomic source of P element transposase in Drosophila melanogaster
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http://hdl.handle.net/2142/218
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| Title: | A stable genomic source of P element transposase in Drosophila melanogaster |
| Author(s): | Robertson, Hugh M.; Preston, Christine R.; Phillis, Randall W.; Johnson-Schlitz, Dena M.; Benz, Wendy K.; Engels, William R. |
| Subject(s): |
Drosophila melanogaster
Genetics |
| Abstract: | A single P element insert in Drosophila melanogoster, called P[ry+A2-3](99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for “helper” plasmids in P element mediated transformation, and can be used to move transformed elements around the genome. |
| Issue Date: | 1988-03 |
| Publisher: | Genetics Society of America |
| Citation Info: | Robertson, H. M., C. R. Preston, R. W. Phillis, D. M. Johnson-Schlitz, W. K. Benz and W. R. Engels 1988. A stable genomic source of P element transposase in Drosophila melanogaster. Genetics 118, 461-470. |
| Genre: | Article |
| Type: | Text |
| Language: | English |
| URI: | http://hdl.handle.net/2142/218 |
| Publication Status: | published or submitted for publication |
| Peer Reviewed: | is peer reviewed |
| Rights Information: | Copyright owned by Genetics Society of America |
| Date Available in IDEALS: | 2007-02-13 |
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