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Title:A stable genomic source of P element transposase in Drosophila melanogaster
Author(s):Robertson, Hugh M.; Preston, Christine R.; Phillis, Randall W.; Johnson-Schlitz, Dena M.; Benz, Wendy K.; Engels, William R.
Subject(s):Drosophila melanogaster
Genetics
Abstract:A single P element insert in Drosophila melanogoster, called P[ry+A2-3](99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for “helper” plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.
Issue Date:1988-03
Publisher:Genetics Society of America
Citation Info:Robertson, H. M., C. R. Preston, R. W. Phillis, D. M. Johnson-Schlitz, W. K. Benz and W. R. Engels 1988. A stable genomic source of P element transposase in Drosophila melanogaster. Genetics 118, 461-470.
Genre:Article
Type:Text
Language:English
URI:http://hdl.handle.net/2142/218
Publication Status:published or submitted for publication
Peer Reviewed:is peer reviewed
Rights Information:Copyright owned by Genetics Society of America
Date Available in IDEALS:2007-02-13


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