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Title:Modified P elements that mimic the P cytotype in Drosophila melanogaster
Author(s):Robertson, Hugh M.; Engels, William R.
Subject(s):Drosophila melanogaster
Genetics
Abstract:Activity of the P family of transposable elements in Drosophila melanogaster is regulated primarily by a cellular condition known as P cytotype. It has been hypothesized that P cytotype depends on a P element-encoded repressoro f transposition and excision. We provide evidence in support of this idea by showing that two modified P elements, each with lesions affecting the fourth transposase exon, mimic most of the P cytotype effects. These elements were identified by means of two sensitive assays capable of detecting repression by a single P element. One assay makes use of cytotype-dependent gene expression of certain P element insertion mutations at the singed bristle locus. The other measures suppressiono f transposase activity from the unusually stable genomic P element, A2-3(99B), that normally produces transposase in both germinal and somatic tissues. The P cytotype-like effects include suppression of snw germline hypermutability, snw somatic mosaicism, pupal lethality, and gonadal dysgenic sterility. Unlike P cytotype, however, there was no reciprocal cross effect in the inheritance of repression.
Issue Date:1989-12
Publisher:Genetics Society of America
Citation Info:Robertson, H. M. and W. R. Engels 1989. Modified P elements that mimic the P cytotype in Drosophila melanogaster. Genetics 123, 815-824.
Genre:Article
Type:Text
Language:English
URI:http://hdl.handle.net/2142/219
Publication Status:published or submitted for publication
Peer Reviewed:is peer reviewed
Rights Information:Copyright owned by Genetics Society of America
Date Available in IDEALS:2007-02-13


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