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Title: | Molecular characterization and induction of a peach beta-1,3-glucanase gene |
Author(s): | Thimmapuram, Jyothi |
Doctoral Committee Chair(s): | Korban, Schuyler S. |
Department / Program: | Biology |
Discipline: | Biology |
Degree Granting Institution: | University of Illinois at Urbana-Champaign |
Degree: | Ph.D. |
Genre: | Dissertation |
Subject(s): | Biology, Molecular
Biology, Plant Physiology |
Abstract: | A peach genomic library of cultivar Stark Earligo was screened using a pea $\beta$-1,3-glucanase gene as a probe. Five clones containing $\beta$-1,3-glucanase sequence were isolated and characterized. A 3.85 Kb DNA fragment of one of the genomic clones, containing peach $\beta$-1,3-glucanase gene was sequenced in both the directions. The peach $\beta$-1,3-glucanase gene, PpGns1, encodes a proenzyme of 350 amino acids, which consists of a 38 amino acid signal peptide and a 312 amino acid mature peptide. This is the longest signal peptide of all plant $\beta$-1,3-glucanase genes so far reported. Putative TATA and CAAT boxes in the 5$\prime$ flanking region and two polyadenylation signals in the 3$\prime$ flanking region were identified. The PpGns1 was induced in in vitro-grown peach shoot cultures following treatment with a culture filtrate of Xanthomonas campestris pv. pruni and ethephon within 12-18 h. However, it was not induced following treatment with mercuric chloride. Characterization of four additional clones revealed two other different types of $\beta$-1,3-glucanase genes in peach. Southern blot analysis of total genomic DNA also indicated that $\beta$-1,3-glucanase gene belongs to a multigene family in peach. |
Issue Date: | 1996 |
Type: | Text |
Language: | English |
URI: | http://hdl.handle.net/2142/22537 |
Rights Information: | Copyright 1996 Thimmapuram, Jyothi |
Date Available in IDEALS: | 2011-05-07 |
Identifier in Online Catalog: | AAI9625202 |
OCLC Identifier: | (UMI)AAI9625202 |
This item appears in the following Collection(s)
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Dissertations and Theses - Biology
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Graduate Dissertations and Theses at Illinois
Graduate Theses and Dissertations at Illinois