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Title:Isolation, characterization and expression of a peach nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II
Author(s):Chung, Kyu Hwan
Doctoral Committee Chair(s):Buetow, Dennis E.; Korban, Schuyler S.
Department / Program:Molecular and Integrative Physiology
Discipline:Molecular and Integrative Physiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Biology, Molecular
Biology, Botany
Biology, Animal Physiology
Abstract:A nuclear gene encoding a light-harvesting chlorophyll a/b-binding polypeptide of photosystem II has been isolated from a peach genomic library (Prunus persica L. cv. Stark Earliglo) and characterized. The genomic library was constructed and screened with a ($\alpha$-$\sp{32}$P) -labeled pea Lhcb1*1 cDNA probe for positive phage containing Lhcb gene sequences. A phage with an Sstl-restricted peach DNA fragment containing an Lhcb gene sequence was identified. This DNA fragment was subcloned (pAB19) and sequenced completely in both directions. pAB19 contains a complete Lhcb gene coding sequence, designated Lhcb1*Pp1, as well as 5$\sp\prime$ and 3$\sp\prime$ flanking regions. Lhcb1*Pp1 encodes a precursor polypeptide of 267 amino acids composed of a transit peptide of 34 amino acids and a mature polypeptide of 233 amino acids. Transcription initiation and termination sites of Lhcb1*Pp1 mRNA were determined by S1 nuclease mapping. The cap site is located 70 bp upstream from the translation start site and 44 bp downstream from a putative TATA box. Multiple polyadenylation sites are present in the 3$\sp\prime$ flanking region. The seasonal expression of Lhcb1*Pp1 was determined by Northern blot and S1 nuclease protection analyses. Both analyses indicate that the relative level of Lhcb1*Pp1 mRNA per unit total leaf RNA declined to a small extent (27%) from the beginning (June) to the end (September) of the growing season, but decreased by 72% with senescence in October. Also, S1 nuclease protection analyses with 5$\sp\prime$ and 3$\sp\prime$ gene probes showed that Lhcb1*Pp1 is expressed in three varieties of P. persica and in light-grown but its expression is undetectable in dark-grown leaves and stems of the Evergreen variety.
Issue Date:1994
Type:Text
Language:English
URI:http://hdl.handle.net/2142/22623
Rights Information:Copyright 1994 Chung, Kyu Hwan
Date Available in IDEALS:2011-05-07
Identifier in Online Catalog:AAI9416348
OCLC Identifier:(UMI)AAI9416348


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