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Title:The structure of cyclic fatty acid monomers isolated from heated linseed oil and their effects on cultured aortic endothelial cells
Author(s):Flickinger, Brent David
Doctoral Committee Chair(s):Perkins, Edward G.
Department / Program:Food Science and Human Nutrition
Discipline:Food Science and Human Nutrition
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Cell
Biology, Animal Physiology
Health Sciences, Nutrition
Abstract:This study reports the complete structural configuration of CFAM isolated from heated linseed oil and the influence of these components in cultured porcine aortic endothelial cells. Using CFAM-oxazoline derivatives, location of unsaturation and ring structures were confirmed using gas chromatography-matrix isolation-Fourier transform infrared spectroscopy and gas chromatography-mass spectrometry. The CFAM were identified as 18-carbon diunsaturated structures containing cyclopentenyl and cyclohexenyl rings in various positions along the molecule. One site of cis unsaturation was always within the ring structure while the other site of unsaturation was either cis or trans and could be located at various positions in either the carboxyl- or methyl-terminal chain of the ring. Three pairs were identified as cyclic stereoisomers.
After incubation with CFAM-containing media for 48 hours, CFAM were incorporated into the membrane phospholipids of cultured endothelial cells. No decrease in endothelial cell density or viability was obsessed. A significant decrease in the activity of calcium ATPase as well as a significant decrease in the integrity of the endothelial cell monolayer were observed following incubation with CFAM-containing media. A decrease in the activity of total ATPase also appeared to be caused by incubation with CFAM.
The incubation of CFAM-containing media caused an increase in the fluidity of the membrane hydrocarbon region as measured by the steady-state anisotropy of DPH. This observed fluidity change may be an artifact caused by DPH incorporation into the intracellular lipid droplets present in CFAM-treated cells. The measurements of the steady-state anisotropy of both TMA-DPH and DPH-PA showed no change in the membrane fluidity.
Endothelial cell growth was shown to be influenced by the presence of fatty-acid free bovine serum albumin, which was used to introduce CFAM into the culture media, and not due to the incorporation of CFAM into endothelial cell membranes. Prostacyclin production was significantly increased in endothelial cells due to incubation with CFAM-BSA compared to BSA alone. The presence of CFAM was either counteracting the inhibitory influence of BSA on PGI$\sb2$ production or directly stimulating PGI$\sb2$ secretion by cultured endothelial cells.
Issue Date:1995
Rights Information:Copyright 1995 Flickinger, Brent David
Date Available in IDEALS:2011-05-07
Identifier in Online Catalog:AAI9543585
OCLC Identifier:(UMI)AAI9543585

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