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Title:Soil colonization and drought adaptation of the actinomycete Frankia
Author(s):Burleigh, Stephen H.
Doctoral Committee Chair(s):Dawson, Jeffrey O.
Department / Program:Biology, Microbiology
Agriculture, Forestry and Wildlife
Discipline:Biology, Microbiology
Agriculture, Forestry and Wildlife
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Biology, Microbiology
Agriculture, Forestry and Wildlife
Abstract:A plant bioassay was used to enumerate infectious Frankia in soil and micro- and molecular techniques were used to identify possible mechanisms by which Frankia survive drying.
Myrica-nodulating Frankia occurred in five Hawaiian volcanic deposits with depositional ages ranging from 20 to 162 years before present. The oldest deposit had a mean estimated nodulation capacity from 450 to 1200 times greater than those of the younger deposits and had increased moisture content, increased organic matter content, and increased vegetative cover, including an abundance of actinorhizal M. faya.
Frankia strains grew more and had greater nodulation capacities after air-desiccation when incubated in sugar osmotica prior to drying. Incubation in 200 mM sucrose, maltose, or melibiose increased the intracellular concentration of the disaccharide trehalose. The intracellular trehalose concentration of Frankia strain HFPArl3 from Alnus rubra was positively and linearly correlated with growth after air-desiccation, suggesting that trehalose production is associated with desiccation tolerance in Frankia.
Anti-dehydrin polyclonal antibodies identified a 60 kD protein in three diverse strains of Frankia. The expression of this protein was independent of osmotic conditions. A wheat dehydrin cDNA weakly hybridized to Frankia genomic DNA. These results provide evidence for the widespread distribution of dehydrin-like proteins in taxonomically diverse organisms. However, there is insufficient evidence to conclude that this protein closely resembles plant dehydrins in form and function.
Axenically-grown Cauarina inoculated with Frankia spores became nodulated only after exposure to airborne contaminants. Casuarina was not nodulated by infectious strains of Frankia when grown under axenic conditions. Manipulation of abiotic and biotic factors did not induce axenic nodulation. Results indicate that the nodulation of Casuarina by Frankia is made possible by unknown airborne abiotic or biotic factors.
Issue Date:1994
Rights Information:Copyright 1994 Burleigh, Stephen H.
Date Available in IDEALS:2011-05-07
Identifier in Online Catalog:AAI9512311
OCLC Identifier:(UMI)AAI9512311

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