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Hepatic gene networks due to the trans-10, cis-12-conjugated linoleic acid supplementation in growing male mice

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Title: Hepatic gene networks due to the trans-10, cis-12-conjugated linoleic acid supplementation in growing male mice
Author(s): Li, Jing
Advisor(s): Loor, Juan L.
Department / Program: Nutritional Sciences
Discipline: Nutritional Sciences
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: M.S.
Genre: Thesis
Subject(s): Conjugated linoleic acid hepatic steatosis
Abstract: Trans-10, cis-12-conjugated linoleic acid (trans-10, cis-12-CLA) has been reported to reduce body fat mass but accompanied by increased liver mass. However, the cellular and molecular mechanisms have been unknown. Our study investigated the effects of trans-10, cis-12-CLA supplementation on hepatic gene expression in growing male mice by measuring mRNA abundance of thirty-eight genes encoding important enzymes, ligand-dependent nuclear receptors, transcription regulators and transporters in lipid metabolism. Feeding growing male mice diets supplemented with 0.3% trans-10, cis-12-CLA for 6 weeks significantly increased the expression of hepatic genes involved in fatty acid uptake (Cd36), TG synthesis (Acaca, Gpam and Scd), lipid droplet formation (Plin2), VLDL assembling/secretion (Mttp and Cideb), ketogenesis and utilization (Hmgcs2 and Bdh1), fatty acid oxidation (Acox1), glyceroneogenesis (Pck1 and Pdk4). The results suggest that the increased liver weight in response to trans-10, cis-12-CLA may be due to increased fatty acid uptake, stimulated TG synthesis, enhanced formation of lipid droplets as well as insufficiently induced VLDL assembly/secretion mechanism in liver. We provided new evidences to advance in the knowledge of the effect of this supplement in a relatively low amount on hepatic gene expression in growing male mice.
Issue Date: 2011-05-25
URI: http://hdl.handle.net/2142/24310
Rights Information: Copyright 2011 Jing Li
Date Available in IDEALS: 2011-05-25
Date Deposited: 2011-05
 

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