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Title:Assembly and analysis of the miscanthus × giganteus transcriptome
Author(s):Barling, Adam R.
Advisor(s):Moose, Stephen P.
Department / Program:Crop Sciences
Discipline:Crop Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:M.S.
Genre:Thesis
Subject(s):Miscanthus
Transcriptome
Sequencing
Biofuel
Bioenergy
Abstract:Miscanthus × giganteus is a sterile triploid hybrid perennial grass that is asexually propagated through rhizome planting. The increasing desire to discover alternative energy sources has recently heightened interest in developing M. × giganteus as a biofuel due to, among other factors, its exceptionally large biomass yield. In this study, mRNA extractions from ten dissimilar M. × giganteus tissues were sequenced, generating over eighty-three million high-quality short paired-end reads. Reads were assembled into over sixty-one thousand contiguous sequences, creating a novel representation of the transcriptome for this prominent non-model organism. Active expression of the ESTs was subsequently analyzed by RNA-Seq on pre-existing Sorghum bicolor gene models in order to generate a series of expression profiles for the Miscanthus tissues sampled. This analysis aims to provide a means for the deeper understanding of M. × giganteus, with the hope that traits such as biomass accumulation, nutrient utilization, and tolerance of environmental stress can be more thoroughly identified and documented.
Issue Date:2011-05-25
URI:http://hdl.handle.net/2142/24444
Rights Information:
Copyright 2011 Adam R. Barling
Date Available in IDEALS:2011-05-25
2013-05-26
Date Deposited:2011-05


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