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Title:Enzymatic diversity in lipoic acid modification of proteins
Author(s):Christensen, Quin H.
Director of Research:Cronan, John E.
Doctoral Committee Chair(s):Cronan, John E.
Doctoral Committee Member(s):Imlay, James A.; Metcalf, William W.; Olsen, Gary J.
Department / Program:Microbiology
Discipline:Microbiology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):lipoic acid
amidotransfer
Bacillus
Thermoplasma
Abstract:Escherichia coli is one of a few organisms with a well characterized and complete metabolic model for lipoic acid metabolism. In this thesis I make discoveries through analysis of the diversity of lipoic acid metabolic genes in other organisms. By examining the distribution and phylogeny of the lipoate ligase protein family, I and others have found gaps and inconsistencies in our knowledge of lipoic acid metabolic pathways. I examine select proteins through heterologous expression in E. coli, allowing complementation studies and biochemical analysis. This enables prediction of lipoic acid metabolism in various organisms and reveals novel pathways and enzymology.
Issue Date:2011-05-25
URI:http://hdl.handle.net/2142/24498
Rights Information:Copyright 2011 Quin H. Christensen
Date Available in IDEALS:2011-05-25
2013-05-26
Date Deposited:2011-05


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