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Bidirectional cargo transport by microtubule-based molecular motors

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Title: Bidirectional cargo transport by microtubule-based molecular motors
Author(s): Hoffman, Melinda
Advisor(s): Selvin, Paul R.
Contributor(s): Selvin, Paul R.; Aksimentiev, Aleksei; Gianetta, Russell W.
Department / Program: Physics
Discipline: Physics
Degree Granting Institution: University of Illinois at Urbana-Champaign
Degree: Ph.D.
Genre: Doctoral
Subject(s): kinesin dynein saltatory motion microtubule molecular motors tug of war
Abstract: We have reconstituted a simple in vitro system using only mammalian dynein and mammalian kinesin attached to a single cargo. These cargoes undergo saltatory motion typically seen in vivo, indicating that the motors engage in a tug-of-war. When the complex hits a barrier, the cargo often reverses direction. In some cases, it tries several up-and-back motions, during which time the dynein likely pulls the cargo onto a different protofilament, and is sometimes able to bypass the blockage. This explains why eliminating kinesin or dynein stops motion in both directions in vivo. We also find that mammalian dynein, but not kinesin, often takes backwards steps when under backward force. However, yeast dynein coupled with mammalian kinesin does not display these attributes, as expected.
Issue Date: 2012-02-01
Genre: thesis
Rights Information: Copyright 2011 Melinda Hoffman
Date Available in IDEALS: 2014-02-01
Date Deposited: 2011-12

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