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Description

Title:Spatiotemporal organization, regulation and function of traction during neutrophil chemotaxis
Author(s):Shin, Myung Eun
Director of Research:Wang, Fei
Doctoral Committee Chair(s):Belmont, Andrew S.
Doctoral Committee Member(s):Wang, Fei; Chen, Jie; Newmark, Phillip A.; Xiang, Yang
Department / Program:Cell & Developmental Biology
Discipline:Cell and Developmental Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Neutrophil Chemotaxis
Traction
Myosin-Light-Chain Kinase
Myosin Type II
Cell Adhesion
Cell Motility
Mechanotransduction
Abstract:Despite recent advances in our understanding of biochemical regulation of neutrophil chemotaxis, little is known about how mechanical factors control neutrophils’ persistent polarity and rapid motility. Here, by using a human neutrophil-like cell line and human primary neutrophils, we describe a dynamic spatiotemporal pattern of tractions in neutrophils during chemotaxis. Tractions are located at both the leading and the trailing edge of neutrophils, where they oscillate with a defined periodicity. Interestingly, traction oscillations at the leading and the trailing edge are out of phase with the tractions at the front leading those at the back, suggesting a temporal mechanism that coordinates leading edge and trailing edge activities. The magnitude and periodicity of tractions depend upon the activity of non-muscle myosin IIA. Specifically, traction development at the leading edge requires myosin light chain kinase (MLCK)-mediated myosin II contractility and is necessary for α5β1-integrin activation and leading edge adhesion. Localized myosin II activation induced by spatially activated small GTPase Rho and its downstream kinase p160-ROCK, as previously reported, leads to contraction of actin-myosin II complexes at the trailing edge, causing it to de-adhere. Our data identify a key biomechanical mechanism for persistent cell polarity and motility.
Issue Date:2012-02-01
URI:http://hdl.handle.net/2142/29509
Rights Information:Copyright 2011 Myung Eun Shin
Date Available in IDEALS:2014-02-01
Date Deposited:2011-12


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