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Biophysical Applications of Single Molecule Fluorescence Localization

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Title: Biophysical Applications of Single Molecule Fluorescence Localization
Author(s): Gordon, Matthew Paul
Doctoral Committee Chair(s): Selvin, P. R.
Department / Program: Physics
Discipline: Physics
Degree: Ph.D.
Genre: Dissertation
Subject(s): Fluorescence Spectroscopy Genetic Haplotypes Photobleaching
Abstract: Fluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photo-bleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.
Issue Date: 2006-10
Genre: Dissertation / Thesis
Type: Text
Language: English
Rights Information: ©2006 Matthew Paul Gordon
Date Available in IDEALS: 2012-10-12
Identifier in Online Catalog: 5639998

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