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Title:Biophysical Applications of Single Molecule Fluorescence Localization
Author(s):Gordon, Matthew Paul
Doctoral Committee Chair(s):Selvin, Paul R.
Department / Program:Physics
Discipline:Physics
Degree:Ph.D.
Genre:Dissertation
Subject(s):Fluorescence Spectroscopy
Genetic Haplotypes
Photobleaching
Abstract:Fluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photo-bleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.
Issue Date:2006-10
Genre:Dissertation / Thesis
Type:Text
Language:English
URI:http://hdl.handle.net/2142/34737
Rights Information:©2006 Matthew Paul Gordon
Date Available in IDEALS:2012-10-12
Identifier in Online Catalog:5639998


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