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Title:High resolution optical DNA mapping
Author(s):Baday, Murat
Director of Research:Selvin, Paul R.
Doctoral Committee Chair(s):Schroeder, Charles M.
Doctoral Committee Member(s):Selvin, Paul R.; Nayfeh, Munir H.; Myong, Su-A; Kuhlman, Thomas E.
Department / Program:School of Molecular & Cell Bio
Discipline:Biophysics & Computnl Biology
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Super-resolution
optical DNA mapping
genomic variations
copy-number variations
Single molecule-High Resolution Imaging with Photobleaching (SHRImP)
High-Resolution Co-localization (SHREC)
Deoxyribonucleic Acid (DNA)
Abstract:Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10–fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.
Issue Date:2013-05-24
URI:http://hdl.handle.net/2142/44261
Rights Information:Copyright 2013 Murat Baday
Date Available in IDEALS:2013-05-24
Date Deposited:2013-05


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