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Title:RNA-SEQ driven analysis of seasonal gene expression variation in miscanthus �� giganteus
Author(s):Barling, Adam R.
Director of Research:Moose, Stephen P.
Doctoral Committee Chair(s):Moose, Stephen P.
Doctoral Committee Member(s):Hudson, Matthew E.; Brown, Patrick J.; Bernacchi, Carl J.
Department / Program:Crop Sciences
Discipline:Crop Sciences
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Ribonucleic Acid Sequencing (RNA-Seq)
Miscanthus
Biofuel
Abstract:Miscanthus × giganteus is a C4 grass that has generated a large amount of interest as a potential biofuel crop due to its high level of biomass production, its perenniality, and its sterility. In this study, two separate M. × giganteus RNA-Seq datasets were generated to help explore the characteristics of M. × giganteus at the level of gene expression: a ten-tissue dataset suitable for examining genes with tissue-preferred expression, and a twenty-four sample dataset for examining the changes in gene expression that occur over the growing season. Aided by these datasets, aspects and potential mediators of M. × giganteus’ seasonal developmental cycle and changes in the utilization, storage, and long-distance mobilization and remobilization of the essential nutrient nitrogen have been studied. These RNA-Seq datasets have been verified with RT-qPCR and compared to amino acid and elemental concentration profiles; as a result, many seasonal changes in gene expression corresponding to the growth and development of M. × giganteus have been documented in order to better define the traits that make this crop such an outstanding biofuel candidate.
Issue Date:2014-01-16
URI:http://hdl.handle.net/2142/46926
Rights Information:Copyright 2013 Adam Robert Barling
Date Available in IDEALS:2014-01-16
2016-01-16
Date Deposited:2013-12


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