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Title:Effects of stiffness gradient and hydrostatic pressure on cell migratory behaviors
Author(s):Joaquin, Danielle
Advisor(s):Hsia, K. Jimmy
Department / Program:Mechanical Sci & Engineering
Discipline:Mechanical Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Tissue Engineering
Biological stiffness gradient
Cell migration
Cell motility
Cellular organization
Pressurized incubation chamber
Scratch Assay
Abstract:This thesis explores the effects of mechanical stimuli on cell patterning and behavior, which may influence our understanding of in vivo processes such as morphogenesis, wound healing, disease progression, tumor formation, and metastatic events. We begin by looking at the effects of a stiffness gradient in three-dimensional culture environment, which builds upon prior research on the effects of stiffness in a two-dimensional culture, but provides a platform for studying these outcomes that is more akin to the in vivo environment. Then we look at the effect of hydrostatic pressure using a non-contact pressure chamber that provides a uniform pressure environment while maintaining physiological conditions needed for cell growth. By seeking to better understand the migratory responses of cells to various mechanical stimuli, we gain tools and knowledge that can be used to aid in diagnoses and treatment of a variety of diseases.
Issue Date:2014-05-30
Rights Information:Copyright 2014 Danielle Joaquin
Date Available in IDEALS:2014-05-30
Date Deposited:2014-05

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