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Title:High-throughput platforms for investigating bioadhesion and cell migration
Author(s):Choi, Sangwook
Director of Research:Leckband, Deborah E.
Doctoral Committee Chair(s):Leckband, Deborah E.
Doctoral Committee Member(s):Higdon, Jonathan J.L.; Braun, Paul V.; Kraft, Mary L.
Department / Program:Chemical & Biomolecular Engr
Discipline:Chemical Engineering
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):polymer gradient
cell migration
wound healing
soft lithography
surface chemistry
Abstract:Engineered in-vitro platforms or patterned biomaterials enabled a wide range of studies that led a deeper understanding of biological processes. This thesis describes the use of surface chemistry and soft lithography that enabled high-throughput analyses of cell adhesion, migration, and wound healing. In this thesis, I investigated engineering design rules that determine the efficiency of thermally tunable cell adhesion and release from poly(N-isopropyl acrylamide) brushes. Large format polymer gradients were used to screen grafting densities and substrate chemistries that alter both cell adhesion and rapid, reversible release by switching temperature. The study demonstrated the interplay between protein adsorption mechanisms, surface chemistry, and polymer properties on the efficacy of thermally controlled, reversible bioadhesion. I also used these large format gradients to generate shallow immobilized protein gradients to investigate the effect of ligand density on cell adhesion and migration. The results showed the utility of these large format gradients for high-throughput analysis of cell adhesion and migration on different extracellular matrix compositions, as an alternative to other gradient generating methodologies. The third main focus of this thesis used a fabricated, micropillar based wound healing platform to conduct high throughput wound healing analyses. With this approach, I investigated the impact of genetic variations on endothelial wound healing relevant to acute lung injury.
Issue Date:2014-09-16
Rights Information:Copyright 2014 Sangwook Choi
Date Available in IDEALS:2014-09-16
Date Deposited:2014-08

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