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Title:Bioenergetics and diffusion in the crowded milieu of living cells
Author(s):Heikal, Ahmed A.
Subject(s):Mini-symposium: Spectroscopy in Kinetics and Dynamics
Abstract:Intracellular nicotinamide adenine dinucleotide (NADH) is a key cofactor in energy metabolism pathways and a myriad of oxidation-reduction reactions in living cells. The crowded milieu of these cells with organelles and macromolecules influences many biological processes such as biomolecular diffusion, protein-protein and protein-substrate interactions, and protein folding. In this contribution, I will highlight our recent findings on the role of macromolecular crowding on biochemical reaction between NADH and selected dehydrogenases in both living cells and in controlled macromolecules-rich environment. In addition, multiscale diffusion (rotational and translational) of a small fluorophore will be used to understand the role of non-specific binding, heterogeneity in microenvironmental viscosity in crowded solutions. Our experimental approach is a combination of fluorescence lifetime imaging microscopy, time-resolved anisotropy and fluorescence correlation spectroscopy. The broader impacts of these results will be discussed within the context of energy metabolism and biophysics in the crowded milieu of living cells.
Issue Date:2014-06-17
Publisher:International Symposium on Molecular Spectroscopy
Citation Info:Heikal, A. Bioenergetics and diffusion in the crowded milieu of living cells. Proceedings of the International Symposium on Molecular Spectroscopy, Urbana, IL June 16-21, 2014.
Genre:Conference Paper / Presentation
Rights Information:Copyright 2014 by the authors. Licensed under a Creative Commons Attribution 4.0 International License.
Date Available in IDEALS:2014-11-21

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