Title: | Bioenergetics and diffusion in the crowded milieu of living cells |
Author(s): | Heikal, Ahmed A. |
Subject(s): | Mini-symposium: Spectroscopy in Kinetics and Dynamics |
Abstract: | Intracellular nicotinamide adenine dinucleotide (NADH) is a key cofactor in energy metabolism pathways and a myriad of oxidation-reduction reactions in living cells. The crowded milieu of these cells with organelles and macromolecules influences many biological processes such as biomolecular diffusion, protein-protein and protein-substrate interactions, and protein folding. In this contribution, I will highlight our recent findings on the role of macromolecular crowding on biochemical reaction between NADH and selected dehydrogenases in both living cells and in controlled macromolecules-rich environment. In addition, multiscale diffusion (rotational and translational) of a small fluorophore will be used to understand the role of non-specific binding, heterogeneity in microenvironmental viscosity in crowded solutions. Our experimental approach is a combination of fluorescence lifetime imaging microscopy, time-resolved anisotropy and fluorescence correlation spectroscopy. The broader impacts of these results will be discussed within the context of energy metabolism and biophysics in the crowded milieu of living cells. |
Issue Date: | 2014-06-17 |
Publisher: | International Symposium on Molecular Spectroscopy |
Citation Info: | Heikal, A. Bioenergetics and diffusion in the crowded milieu of living cells. Proceedings of the International Symposium on Molecular Spectroscopy, Urbana, IL June 16-21, 2014. |
Genre: | Conference Paper / Presentation |
Type: | Text |
Language: | English |
URI: | http://hdl.handle.net/2142/55863 |
Rights Information: | Copyright 2014 by the authors. Licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0/ |
Date Available in IDEALS: | 2014-11-21 |