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Title:IL-4 Western Blot Analysis of High Fat Diet and Control Diet Sprague Dawley Rats
Author(s):Mitchell, Emmanuel; Kriska, Adam; Pan, Yuan-Xiang
maternal programming
Abstract:The Lowry Protein Assay is a fundamental process in the ultimate procedure of Western Blotting; it is used to determine the protein content of a solution, which is essential in calculating the necessary concentration of antibodies. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels were prepared with a 12% resolving gel and 5% stacking gel. After running the SDS-PAGE, we performed a western blot analysis on our samples. Western blot results showed that there was a significant difference between IL-4 expression in our two different animal experimental groups. Animals with a maternal high fat diet showed increased expression of IL-4, but animals with a maternal control diet showed lower expressions of IL-4. Methylated DNA immunoprecipitation also confirmed our results and showed a region of hypermethylation upstream of the IL-4 gene in the control group.
Issue Date:2014
Citation Info:Mitchell, E., A. Kriska, and Y. Pan. 2014. IL-4 Western Blot Analysis of High Fat Diet and Control Diet Sprague Dawley Rats. i-ACES 1(1), 86-90.
Rights Information:Copyright 2014 Emmanuel Mitchell, Adam Kriska, Yuan-Xiang Pan
Date Available in IDEALS:2014-12-12

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