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Title:Cellular Immunity in Hamsters After Immunization With Vaccinia Virus: Studies on Macrophage Functional Heterogeneity and Interactions Between Natural Killer Cells and Macrophages
Author(s):Chapes, Stephen Keith
Department / Program:Veterinary Medical Science
Discipline:Veterinary Medical Science
Degree Granting Institution:University of Illinois at Urbana-Champaign
Subject(s):Health Sciences, Immunology
Abstract:This study demonstrated that macrophage (M(phi)) subpopulations can be isolated from normal and vaccinia virus-immune peritoneal exudate cells after separation on bovine serum albumin (BSA) discontinuous density gradients. The M(phi) subpopulations from normal and early- or late-immune animals were all phagocytic but differed in their ability to mediate cytotoxicity. Normal M(phi) subpopulations were not cytotoxic, whereas the 40/30 and pellet fractions collected from BSA gradients of immune M(phi)s were cytotoxic. In contrast, M(phi)s isolated from the lighter fractions were found to be poor killer cells.
This investigation demonstrated that immune peritoneal M(phi)s could activate bone marrow (BM) natural killer (NK) cells in vitro. Freshly harvested, noncytotoxic, BM cells were co-cultured with immune M(phi)s for 20 to 24 hours and were thereafter assayed for cytotoxicity in a ('51)Cr release assay. We found that co-cultured BM cells became potent killer cells. These activated BM cells were characterized as being nonspecifically cytotoxic, nylon wool nonadherent, Fc-receptor positive and radiation resistant. In addition, it became apparent that helper M(phi)s are generated sooner after immunization with vaccinia virus, than cytotoxic M(phi)s.
Only two BSA-Subpopulations of early-immune M(phi)s were found to mediate helper function. Forty-thirty and 30/20 M(phi)s were both able to generate BM-NK cells whereas the other subpopulations lacked helper activity.
Our findings suggest that BM-NK cell activation by the M(phi) is probably mediated through M(phi)-BM-NK contact. Interferon was undetected in our co-culture supernatants and other soluble mediators were also unable to stimulate freshly harvested BM cells.
This study discusses a model for the ontogeny of NK cells, and the role of M(phi)s and interferon in the generation of fully mature NK cells.
Issue Date:1981
Description:111 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1981.
Other Identifier(s):(UMI)AAI8114398
Date Available in IDEALS:2014-12-14
Date Deposited:1981

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