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|Title:||The Influence of Age and Sampling Depth on Muscle Fiber Type Percentages and Enzyme Activities of Equine Middle Gluteal Muscle|
|Author(s):||Kline, Kevin Halden|
|Department / Program:||Animal Science|
|Degree Granting Institution:||University of Illinois at Urbana-Champaign|
|Subject(s):||Agriculture, Animal Culture and Nutrition|
|Abstract:||The effects of age and sampling depth on histochemical and biochemical properties of equine gluteus medius were examined.
Percent FG and SO fibers of equine gluteus medius greatly decreased and increased, respectively, as a function of increasing sampling depth. Percent FOG fibers decreased slightly in deeper regions of the muscle.
Activities of both phosphorylase and lactate dehydrogenase were 50 percent lower in samples obtained 8 cm below the middle gluteal superficial fascia when compared to samples 1 cm below the fascia. Citrate synthase and 3-hydroxyacyl-CoA dehydrogenase activities were several fold higher at a sampling depth of 8 centimeters compared to the 1 centimeter sampling depth. The need to carefully document intramuscular metabolic variation and sampling site in equine muscle biopsy studies was demonstrated.
Percent FOG fibers of gluteus medius decreased from 1 day to 6 months of age, then tended to increase through 1 year of age. Percent FG and SO fibers increased from 1 day of age through 3 to 6 months of age, then stabilized or slightly decreased. Only small changes in histochemical staining patterns were detected as a function of age from 1 day to 1 year of age in horses.
Activities of phosphorylase and lactate dehydrogenase showed large increases in equine gluteus medius from 1 day to 6 months of age, then slightly declined. Middle gluteal citrate synthase and 3-hydroxyacyl-CoA dehydrogenase activities tended to decrease during the first 3 to 6 months of age. Enzymes associated with glycolytic metabolism had large increases in activity from birth to 6 months of age.
Enzymatic changes in equine gluteus medius were large as a function of both sampling depth and age. Histochemical changes were large with respect to sampling depth; however, only small changes were found with age. Enzyme analyses were found more useful for assessment of muscle metabolism.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1987.
|Date Available in IDEALS:||2014-12-15|