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Title:Analytical Chemiluminescence in Microporous Membrane Flow Cells
Author(s):Pilosof, David
Department / Program:Chemistry
Discipline:Chemistry
Degree Granting Institution:University of Illinois at Urbana-Champaign
Degree:Ph.D.
Genre:Dissertation
Subject(s):Chemistry, Analytical
Abstract:Microporous membrane chemiluminescence (CL) flow cells are presented as an advantageous method for chemical reagent and analyte mixing in continuous flow. The method adds simplicity, significant reagent economy and minimal sample contamination to the conventional advantages of analytical CL determinations.
The most significant application of the proposed method is in coupling enzymatic reactions that generate hydrogen peroxide to CL detection, in the same flow cell, in spite of the very different pH optima required by both processes. The analysis of glucose, using this approach, is studied in depth, and results obtained are compared to values determined by a routine assay method with good correlation. Other applications include analysis by use of other oxidase enzymes, determination of mixtures and determination of sugars that can be enzymatically converted to D-glucose.
Issue Date:1982
Type:Text
Description:175 p.
Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1982.
URI:http://hdl.handle.net/2142/70204
Other Identifier(s):(UMI)AAI8302961
Date Available in IDEALS:2014-12-15
Date Deposited:1982


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